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Reverse transcriptase PCR amplification of Rickettsia typhi from infected mammalian cells and insect vectors
(American Society for Microbiology, 1998-06)
We developed a reverse transcriptase PCR assay to detect expression of 120- and 17-kDa antigen genes in Rickettsia typhi. Infected Vero cell and flea RNAs were reverse transcribed by using random hexamers. The cDNA was ...
Quantitation of Plasmodium falciparum sporozoites transmitted in vitro by experimentally infected Anopheles gambiae and Anopheles stephensi
(American Society of Tropical Medicine and Hygiene, 1991)
The frequency and numbers of Plasmodium falciparum sporozoites transmitted in vitro and corresponding sporozoite loads were determined for experimentally infected Anopheles gambiae and An. stephensi. Geometric mean (GM) ...
Sporogonic development of cultured Plasmodium falciparum in six species of laboratory-reared Anopheles mosquitoes
(American Society of Tropical Medicine and Hygiene, 1994)
Sporogonic development of cultured Plasmodium falciparum was compared in six species of Anopheles mosquitoes. A reference species, A. gambiae, was selected as the standard for comparison. Estimates of absolute densities ...
Plasmodium falciparum: The population structure of mature gametocyte cultures has little effect on their innate fertility
(Elsevier BV, 1994)
In vitro cultured Plasmodium falciparum gametocytes were fed to Anopheles gambiae (G3) mosquitoes to identify parasite population characteristics useful for predicting successful mosquito infections. Parameters were collected ...
Exflagellation responses of cultured Plasmodium falciparum (Haemosporida: Plasmodiidae) gametocytes to human sera and midguts of anopheline mosquitoes (Diptera: Culicidae)
(Oxford University Press (OUP), 1994)
The process of exflagellation was quantified for cultured Plasmodium falciparum gametocytes exposed to human sera and midgut homogenates from six vector species of Anopheles mosquitoes. Neither serum factors related to ...
Knowledge, attitudes, and practices regarding ticks, tick-borne pathogens, and tick prevention among beef producers in Oklahoma
(Society of Southwestern Entomologists, 2020-06)
Tick-borne diseases are increasing in the United States, with regional need to understand how knowledge of ticks translates into preventative behavior among specific occupational groups. Little is known regarding what ...
Immunological factor that affects Anopheles gambiae survival
(The American Mosquito Control Association, 1995)
High titers of antibodies against Anopheles gambiae midguts were produced in New Zealand rabbits to identify midgut targets for an antimosquito vaccine. The serum from one of 8 rabbits (designated R2B6) killed 71.6% (Abbott's ...
Mycobacteria, metals, and the macrophage
(Wiley, 2015-03)
Mycobacterium tuberculosis is a facultative intracellular pathogen that thrives inside host macrophages. A key trait of M. tuberculosis is to exploit and manipulate metal cation trafficking inside infected macrophages to ...
Invasive plants as foci of mosquito-borne pathogens: Red cedar in the southern Great Plains of the USA
(Springer Science and Business Media LLC, 2021-10-05)
West Nile virus (WNV) is the most significant mosquito-borne disease affecting humans in the United States. Eastern redcedar (ERC) is a native encroaching plant in the southern Great Plains that greatly alters abiotic ...
Protein complex from human milk enhances the activity of antibiotics and drugs against Mycobacterium tuberculosis.
(American Society for Microbiology, 2019-02)
Mycobacterium tuberculosis, the causative agent of human tuberculosis (TB), has surpassed HIV/AIDS as the leading cause of death from a single infectious agent. The increasing occurrence of drug-resistant strains has become ...