Reverse transcriptase PCR amplification of Rickettsia typhi from infected mammalian cells and insect vectors
Date
1998-06Author
Higgins, James A.
Radulovic, Suzana
Noden, Bruce H.
Troyer, Jill M.
Azad, Abdu F.
Metadata
Show full item recordAbstract
We developed a reverse transcriptase PCR assay to detect expression of 120- and 17-kDa antigen genes in Rickettsia typhi. Infected Vero cell and flea RNAs were reverse transcribed by using random hexamers. The cDNA was amplified by using high concentrations of primer and template in an inexpensive, nonradioactive assay.
Citation
Higgins, J. A., Radulovic, S., Noden, B. H., Troyer, J. M., & Azad, A. F. (1998). Reverse transcriptase PCR amplification of Rickettsia typhi from infected mammalian cells and insect vectors. Journal of Clinical Microbiology, 36(6), pp. 1793-1794. https://doi.org/10.1128/jcm.36.6.1793-1794.1998