dc.contributor.author | Higgins, James A. | |
dc.contributor.author | Radulovic, Suzana | |
dc.contributor.author | Noden, Bruce H. | |
dc.contributor.author | Troyer, Jill M. | |
dc.contributor.author | Azad, Abdu F. | |
dc.date.accessioned | 2022-04-12T13:56:25Z | |
dc.date.available | 2022-04-12T13:56:25Z | |
dc.date.issued | 1998-06 | |
dc.identifier | oksd_noden_reversetranscriptasePCR_1998 | |
dc.identifier.citation | Higgins, J. A., Radulovic, S., Noden, B. H., Troyer, J. M., & Azad, A. F. (1998). Reverse transcriptase PCR amplification of Rickettsia typhi from infected mammalian cells and insect vectors. Journal of Clinical Microbiology, 36(6), pp. 1793-1794. https://doi.org/10.1128/jcm.36.6.1793-1794.1998 | |
dc.identifier.uri | https://hdl.handle.net/11244/335207 | |
dc.description.abstract | We developed a reverse transcriptase PCR assay to detect expression of 120- and 17-kDa antigen genes in Rickettsia typhi. Infected Vero cell and flea RNAs were reverse transcribed by using random hexamers. The cDNA was amplified by using high concentrations of primer and template in an inexpensive, nonradioactive assay. | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.publisher | American Society for Microbiology | |
dc.relation.ispartof | Journal of Clinical Microbiology, 36 (6) | |
dc.relation.uri | https://www.ncbi.nlm.nih.gov/pubmed/9620425 | |
dc.rights | This material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information. | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Antigens, Bacterial | |
dc.subject.mesh | Antigens, Surface | |
dc.subject.mesh | Chlorocebus aethiops | |
dc.subject.mesh | DNA, Complementary | |
dc.subject.mesh | Gene Expression | |
dc.subject.mesh | Insect Vectors | |
dc.subject.mesh | Polymerase Chain Reaction | |
dc.subject.mesh | RNA, Messenger | |
dc.subject.mesh | Rickettsia typhi | |
dc.subject.mesh | Siphonaptera | |
dc.subject.mesh | Vero Cells | |
dc.title | Reverse transcriptase PCR amplification of Rickettsia typhi from infected mammalian cells and insect vectors | |
dc.date.updated | 2022-04-07T14:50:28Z | |
osu.filename | oksd_noden_reversetranscriptasePCR_1998.pdf | |
dc.description.peerreview | Peer reviewed | |
dc.identifier.doi | 10.1128/jcm.36.6.1793-1794.1998 | |
dc.description.department | Entomology and Plant Pathology | |
dc.type.genre | Article | |
dc.type.material | Text | |
dc.subject.keywords | Biodefense | |
dc.subject.keywords | Vector-Borne Diseases | |
dc.subject.keywords | Emerging Infectious Diseases | |
dc.subject.keywords | Prevention | |
dc.subject.keywords | Biotechnology | |
dc.subject.keywords | Genetics | |
dc.subject.keywords | Rare Diseases | |
dc.subject.keywords | Vaccine Related | |
dc.subject.keywords | Infectious Diseases | |
dc.subject.keywords | 06 Biological Sciences | |
dc.subject.keywords | 07 Agricultural and Veterinary Sciences | |
dc.subject.keywords | 11 Medical and Health Sciences | |
dc.subject.keywords | Microbiology | |
dc.identifier.author | ScopusID: 57213585907 (Higgins, JA) | |
dc.identifier.author | ScopusID: 57194023662 (Radulovic, S) | |
dc.identifier.author | ORCID: 0000-0002-0096-370X (Noden, BH) | |
dc.identifier.author | ScopusID: 6601968347 (Noden, BH) | |
dc.identifier.author | ScopusID: 6701914406 (Troyer, JM) | |
dc.identifier.author | ScopusID: 7102313565 (Azad, AF) | |