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Reverse transcriptase PCR amplification of Rickettsia typhi from infected mammalian cells and insect vectors

dc.contributor.authorHiggins, James A.
dc.contributor.authorRadulovic, Suzana
dc.contributor.authorNoden, Bruce H.
dc.contributor.authorTroyer, Jill M.
dc.contributor.authorAzad, Abdu F.
dc.date.accessioned2022-04-12T13:56:25Z
dc.date.available2022-04-12T13:56:25Z
dc.date.issued1998-06
dc.identifieroksd_noden_reversetranscriptasePCR_1998
dc.identifier.citationHiggins, J. A., Radulovic, S., Noden, B. H., Troyer, J. M., & Azad, A. F. (1998). Reverse transcriptase PCR amplification of Rickettsia typhi from infected mammalian cells and insect vectors. Journal of Clinical Microbiology, 36(6), pp. 1793-1794. https://doi.org/10.1128/jcm.36.6.1793-1794.1998
dc.identifier.urihttps://hdl.handle.net/11244/335207
dc.description.abstractWe developed a reverse transcriptase PCR assay to detect expression of 120- and 17-kDa antigen genes in Rickettsia typhi. Infected Vero cell and flea RNAs were reverse transcribed by using random hexamers. The cDNA was amplified by using high concentrations of primer and template in an inexpensive, nonradioactive assay.
dc.formatapplication/pdf
dc.languageen_US
dc.publisherAmerican Society for Microbiology
dc.relation.ispartofJournal of Clinical Microbiology, 36 (6)
dc.relation.urihttps://www.ncbi.nlm.nih.gov/pubmed/9620425
dc.rightsThis material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information.
dc.subject.meshAnimals
dc.subject.meshAntigens, Bacterial
dc.subject.meshAntigens, Surface
dc.subject.meshChlorocebus aethiops
dc.subject.meshDNA, Complementary
dc.subject.meshGene Expression
dc.subject.meshInsect Vectors
dc.subject.meshPolymerase Chain Reaction
dc.subject.meshRNA, Messenger
dc.subject.meshRickettsia typhi
dc.subject.meshSiphonaptera
dc.subject.meshVero Cells
dc.titleReverse transcriptase PCR amplification of Rickettsia typhi from infected mammalian cells and insect vectors
dc.date.updated2022-04-07T14:50:28Z
osu.filenameoksd_noden_reversetranscriptasePCR_1998.pdf
dc.description.peerreviewPeer reviewed
dc.identifier.doi10.1128/jcm.36.6.1793-1794.1998
dc.description.departmentEntomology and Plant Pathology
dc.type.genreArticle
dc.type.materialText
dc.subject.keywordsBiodefense
dc.subject.keywordsVector-Borne Diseases
dc.subject.keywordsEmerging Infectious Diseases
dc.subject.keywordsPrevention
dc.subject.keywordsBiotechnology
dc.subject.keywordsGenetics
dc.subject.keywordsRare Diseases
dc.subject.keywordsVaccine Related
dc.subject.keywordsInfectious Diseases
dc.subject.keywords06 Biological Sciences
dc.subject.keywords07 Agricultural and Veterinary Sciences
dc.subject.keywords11 Medical and Health Sciences
dc.subject.keywordsMicrobiology
dc.identifier.authorScopusID: 57213585907 (Higgins, JA)
dc.identifier.authorScopusID: 57194023662 (Radulovic, S)
dc.identifier.authorORCID: 0000-0002-0096-370X (Noden, BH)
dc.identifier.authorScopusID: 6601968347 (Noden, BH)
dc.identifier.authorScopusID: 6701914406 (Troyer, JM)
dc.identifier.authorScopusID: 7102313565 (Azad, AF)


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