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The isolation and characterization of phosphatases from the Ehrlich ascites tumor cell line /
(1985)
Peak II was found to have low specificity toward phosphotyrosine but did contain Mg('2+)- or Mn('2+)-dependent p-nitrophenyl phosphate phosphatase activity. Both Peak I and Peak II p-nitrophenyl phosphate phosphatases ...
Testing recently-developed molecular dynamics protocols for structure prediction of polypeptides and prion proteins.
(2007)
Two MD protocols were recently developed that allow a polypeptide to search the PE surface in search of the global PE minimum, which should correspond to the experimental structure. alpha-helical secondary structures have ...
Sequencing and comparative genomics of Leptospira interrogans serovar pomona and Leptospira kirschneri serovar grippotyphosa.
(2007)
Virulence genes that include methyl-accepting chemotaxis protein, flagellar basal body-associated protein, flagellar motor switch protein, Lig A protein, thermolysin, multiple antibiotic resistance protein, acriflavine ...
BACTERIOPHAGE NANOFIBERS ENABLED NANOSTRUCTURES AND TARGET-RECOGNITION FOR BIO-APPLICATIONS
(2015)
Nanostructure is an object system that has dimensions on the nanoscale, i.e. between 0.1 and 100 nanometers. It includes nanotextured surface, nanotube, nanorod, nanofilament, nanoparticle, etc. The field of nanostructure ...
Studies on selected enzymes of the alpha-aminoadipate pathway for lysine biosynthesis in Saccharomyces cerevisiae.
(2005)
Homocitrate synthase (HCS) catalyzes one of the regulated steps of the alpha-aminoadipate pathway for Lys biosynthesis in fungi. His-tagged HCS from Saccharomyces cerevisiae was purified to about 98% using a Ni-NTA resin ...
Low-molecular-weight branched polyethylenimines: Multifunctional beta-lactam potentiators against resistant Gram-negative pathogens
(2022-05-13)
Antibiotic resistance is a simmering threat to the global public health system. Currently, it is estimated by the Centers for Disease Control and Prevention that at least 2.8 million people in the US get infected with ...
Characterization of the protein-protein interactions of the type-II toxin-antitoxin system ParDE1 from Pseudomonas aeruginosa
(2023-05-12)
The emergence of multidrug-resistant bacteria has led to an ever-growing antibiotic resistance crisis. To combat this growing crisis, new strategies for controlling bacterial cell growth must be developed. An underdeveloped ...
Ferric enterobactin transport through FepA in Escherichia coli: From hydrophobic stacking to charge interactions.
(1999)
The site-directed alanine substitution studies revealed that three aromatic residues in the central region of FepA, Y260, Y272, and F329, are involved in the binding and transport of ferric enterobactin. A significant ...
A comparison of the standard DNA shotgun sequencing approach and the exponential DNA amplification method for sequencing BAC, fosmid, plasmid, and mitochondrial DNA.
(2003)
Therefore, during the initial phase of my studies, I became well versed in the standard shotgun sequencing techniques and completed the sequence of six regions of the human and mouse genome totaling over 1 Mbp. Then, because ...