KINETIC DOPING OF SILICA SOL-GEL THIN FILMS AND APPLICATION AS BIOSENSORS

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dc.contributor.advisorYip, Wai Tak
dc.contributor.authorCrosley, Matthew
dc.contributor.committeeMemberHansmann, Ulrich
dc.contributor.committeeMemberRice, Charles
dc.contributor.committeeMemberWhite, Robert
dc.contributor.committeeMemberBumm, Lloyd
dc.date.accessioned2019-04-02T18:23:19Z
dc.date.available2019-04-02T18:23:19Z
dc.date.issued2019-05-10
dc.date.manuscript2019-03
dc.description.abstractEasy to use and easy to produce biosensors would have a huge range of applications. To reach this goal many see the incorporation of a protein into a sol-gel network as one of the most viable options. The current most prevalent technique of pre-doping presents inherent limits on the concentration possible for the resulting thin film. In this study we demonstrate a new process utilizing the newly developed kinetic doping method to load silica sol-gel thin films with cytochrome C (CytC) and horseradish peroxidase (HRP). Both enzymes are shown to successfully load and have a concentration increase over their original loading solution by factors of 1300X and 2600X, respectively. Furthermore, each enzyme once loaded retained the ability to act as a catalyst for the detection of hydrogen peroxide. A new methodology for adapting the Bradford assay to determining the actual amount of accessible enzyme loaded is created and utilized. Ultimately the CytC- and HRP-loaded thin films were found to have enzyme concentrations of 11±1 mM and 6.0±0.4 mM, respectively, a 6X increase in concentration over a sample made via existing post-doping techniques under the same conditions.en_US
dc.identifier.urihttps://hdl.handle.net/11244/317832
dc.languageen_USen_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subjectChemistry, Physical.en_US
dc.thesis.degreePh.D.en_US
dc.titleKINETIC DOPING OF SILICA SOL-GEL THIN FILMS AND APPLICATION AS BIOSENSORSen_US
ou.groupCollege of Arts and Sciences::Department of Chemistry and Biochemistryen_US
shareok.orcidhttps://orcid.org/0000-0001-8217-5653en_US

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