M13 BACTERIOPHAGE-BASED POLYMERS, A PROMISING NEXT GENERATION OF BIOHYDROGEL

Abstract

Hydrogel has been considered a promising material for many biomedical applicational researches due to its unique properties. One such promising hydrogel is a peptide-based hydrogel due to its excellent biocompatibility and bioinjectability, which are important properties for most biomedical applicative research. However, synthesis of peptide is a biohazardous, difficult, and expensive process. M13 phage can be a better alternative biomaterial as a monomer for constructing a good hydrogel because of easy amplification process, cost efficiency, and safe, bio-green chemical production. In this thesis article, copper-M13 phage hydrogel shows a good cytotoxicity to breast cancer cell lines via pH-controlled release of copper (II) ion-phage complex at body temperature 37oC. Gold (III)-M13 phage hydrogel has been constructed via metal-beta amyloid peptide interaction in a stable form in various aqueous biochemical media. It induces fine gold nanoparticle synthesis after the reduction of gold (III) ion by phages in the hydrogel. M13 phage-based hydrogel has a great potential to become a next generation biopolymer in the near future.