| dc.contributor.advisor | Clinkenbeard, Kenneth D. | |
| dc.contributor.author | Mani, Rinosh Joshua | |
| dc.date.accessioned | 2014-04-16T03:15:58Z | |
| dc.date.available | 2014-04-16T03:15:58Z | |
| dc.date.issued | 2006-07-01 | |
| dc.identifier.uri | https://hdl.handle.net/11244/9821 | |
| dc.description.abstract | Using the bi-cell surface plasmon resonance instrument, we were able to detect thrombin in the concentration range of 500 femtomoles to 2 picomoles with thrombin aptamer as the capture probe. The lowest limit of detection was 250 femtomoles. The affinity of the aptamer for prothrombin was also investigated, which was detected at 2 picomoles. Using serum samples, 1 picomole of thrombin was detected. We proved that an aptamer could be used as a capture ligand in bi-cell SPR instrument. This model could be used in a clinical setting for the real time detection of diagnostically relevant biomolecules. | |
| dc.format | application/pdf | |
| dc.language | en_US | |
| dc.publisher | Oklahoma State University | |
| dc.rights | Copyright is held by the author who has granted the Oklahoma State University Library the non-exclusive right to share this material in its institutional repository. Contact Digital Library Services at lib-dls@okstate.edu or 405-744-9161 for the permission policy on the use, reproduction or distribution of this material. | |
| dc.title | Application of Bi-cell Surface Plasmon Resonance for the Detection of Aptamer Mediated Thrombin Capture in Serum | |
| dc.type | text | |
| dc.contributor.committeeMember | Meinkoth, James H. | |
| dc.contributor.committeeMember | Morton, Rebecca J. | |
| osu.filename | Mani_okstate_0664M_1949.pdf | |
| osu.college | Center for Veterinary Health Sciences | |
| osu.accesstype | Open Access | |
| dc.description.department | Veterinary Pathobiology | |
| dc.type.genre | Thesis | |
