Hsp90 Interactome
Abstract
The purpose of this study was to confirm identify of Armadillo Repeat Chaperone Binding Protein 2 (ARCBP2) and interaction with ARCBP2 and Hsp90; to characterize 15-lipoxygenase-1 (15-LOX-1) interaction with Hsp90. Pull-down assays, Western blotting and Mass spectrum fingerprinting assay determined the association between Hsp90 and proteins. The mechanism of inhibition of Hsp90 by drug geldanamycin and molybdate in vitro was studied using coupled transcription/translation in nuclease reticulocyte lysate, and was analyzed using pull-down assays, Western blotting and autoradiography. Hsp90-dependent signaling pathway were examined in K562 cell using transfection by treating with drug geldanamycin, followed by immunoadsorption and Western blotting.
Collections
- OSU Theses [15752]