dc.contributor.advisor | Kaul, Rashmi | |
dc.contributor.author | Bolt, Brittany R. | |
dc.date.accessioned | 2014-04-15T20:25:27Z | |
dc.date.available | 2014-04-15T20:25:27Z | |
dc.date.issued | 2010-07-01 | |
dc.identifier.uri | https://hdl.handle.net/11244/8904 | |
dc.description.abstract | The purpose of the present study was to investigate the role of estrogen in modulating TNF-α responses during Dr+ E.coli infection in mIMCD-3 cells. E2 and ER antagonist ICI-182780 pre-treated mIMCD-3 cells were infected with Dr+ E.coli for 2 and 8 hours. TNF-α and DAF expression at mRNA levels were determined by quantitative Real-time RT-PCR and secreted protein quantified by ELISA. E2 modulated TNF-α mRNA levels at both time points in Dr+ E.coli infected mIMCD-3 cells. ICI-182, 780 and E2 co-treatment of cells reversed the observed E2 effects on TNF-α mRNA. TNF-α mRNA increase in DR+ E.coli infected cells also coincided with down-regulation of DAF mRNA. Significant reversal of DAF mRNA levels was induced by co-treatment of the cells with E2 and ICI. Thus, E2 mediates protective effects against Dr+ E.coli invasion in mIMCD-3 cells by modulating TNF-α and DAF production. | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.publisher | Oklahoma State University | |
dc.rights | Copyright is held by the author who has granted the Oklahoma State University Library the non-exclusive right to share this material in its institutional repository. Contact Digital Library Services at lib-dls@okstate.edu or 405-744-9161 for the permission policy on the use, reproduction or distribution of this material. | |
dc.title | Estrogen Regulation of Tumor Necrosis Factor Alpha During Dr+ Escherichia Coli Uropathogenesis | |
dc.type | text | |
dc.contributor.committeeMember | Rouch, Alexander | |
dc.contributor.committeeMember | Curtis, Kathleen | |
osu.filename | Bolt_okstate_0664M_11072.pdf | |
osu.college | Agricultural Sciences and Natural Resources | |
osu.accesstype | Open Access | |
dc.description.department | Department of Biochemistry and Molecular Biology | |
dc.type.genre | Thesis | |
dc.subject.keywords | microbiology | |