dc.contributor.author | Mitra, Avishek | |
dc.contributor.author | Fay, Pamela A. | |
dc.contributor.author | Vendura, Khoury W. | |
dc.contributor.author | Alla, Zimrisha | |
dc.contributor.author | Carroll, Ronan K. | |
dc.contributor.author | Shaw, Lindsey N. | |
dc.contributor.author | Riordan, James T. | |
dc.date.accessioned | 2023-02-16T16:48:10Z | |
dc.date.available | 2023-02-16T16:48:10Z | |
dc.date.issued | 2014-08 | |
dc.identifier | oksd_mitra_dependent_control_of_acid_2014 | |
dc.identifier.citation | Mitra, A., Fay, P. A., Vendura, K. W., Alla, Z., Carroll, R. K., Shaw, L. N., Riordan, J. T. (2014). σᴺ-dependent control of acid resistance and the locus of enterocyte effacement in enterohemorrhagic Escherichia coli is activated by acetyl phosphate in a manner requiring flagellar regulator FlhDC and the σS antagonist FliZ. MicrobiologyOpen, 3(4), 497-512. https://doi.org/10.1002/mbo3.183 | |
dc.identifier.issn | 2045-8827 | |
dc.identifier.uri | https://hdl.handle.net/11244/337042 | |
dc.description.abstract | In enterohemorrhagic Escherichia coli (EHEC), sigma factor N (σᴺ) regulates glutamate-dependent acid resistance (GDAR) and the locus of enterocyte effacement (LEE); discrete genetic systems that are required for transmission and virulence of this intestinal pathogen. Regulation of these systems requires nitrogen regulatory protein C, NtrC, and is a consequence of NtrC-σᴺ-dependent reduction in the activity of sigma factor S (σS). This study elucidates pathway components and stimuli for σᴺ-directed regulation of GDAR and the LEE in EHEC. Deletion of fliZ, the product of which reduces σS activity, phenocopied rpoN (σᴺ) and ntrC null strains for GDAR and LEE control, acid resistance, and adherence. Upregulation of fliZ by NtrC-σᴺ was shown to be indirect and required an intact flagellar regulator flhDC. Activation of flhDC by NtrC-σᴺ and FlhDC-dependent regulation of GDAR and the LEE was dependent on σᴺ-promoter flhDₚ₂, and a newly described NtrC upstream activator sequence. Addition of ammonium chloride significantly altered expression of GDAR and LEE, acid resistance, and adherence, independently of rpoN, ntrC, and the NtrC sensor kinase, ntrB. Altering the availability of NtrC phosphodonor acetyl phosphate by growth without glucose, with acetate addition, or by deletion of acetate kinase ackA, abrogated NtrC-σᴺ-dependent control of flhDC, fliZ, GDAR, and the LEE. | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.publisher | Wiley | |
dc.relation.ispartof | MicrobiologyOpen, 3 (4) | |
dc.rights | This material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information. | |
dc.subject.mesh | carboxylic acids | |
dc.subject.mesh | drug tolerance | |
dc.subject.mesh | enterohemorrhagic Escherichia coli | |
dc.subject.mesh | Escherichia coli proteins | |
dc.subject.mesh | gene deletion | |
dc.subject.mesh | gene expression regulation, bacterial | |
dc.subject.mesh | glutamates | |
dc.subject.mesh | organophosphates | |
dc.subject.mesh | phosphoproteins | |
dc.subject.mesh | repressor proteins | |
dc.subject.mesh | sigma factor | |
dc.subject.mesh | trans-activators | |
dc.subject.mesh | transcriptional activation | |
dc.title | σᴺ-dependent control of acid resistance and the locus of enterocyte effacement in enterohemorrhagic Escherichia coli is activated by acetyl phosphate in a manner requiring flagellar regulator FlhDC and the σS antagonist FliZ | |
dc.date.updated | 2023-02-15T23:01:25Z | |
dc.note | open access status: Gold OA | |
osu.filename | oksd_mitra_dependent_control_of_acid_2014.pdf | |
dc.identifier.doi | 10.1002/mbo3.183 | |
dc.description.department | Microbiology and Molecular Genetics | |
dc.type.genre | Article | |
dc.type.material | Text | |
dc.subject.keywords | prevention | |
dc.subject.keywords | infectious diseases | |
dc.subject.keywords | infection | |
dc.subject.keywords | acid resistance | |
dc.subject.keywords | sigma factor N | |
dc.subject.keywords | microbiology | |
dc.identifier.author | ORCID: 0000-0003-0243-2045 (Mitra, Avishek) | |
dc.identifier.essn | 2045-8827 | |