σᴺ-dependent control of acid resistance and the locus of enterocyte effacement in enterohemorrhagic Escherichia coli is activated by acetyl phosphate in a manner requiring flagellar regulator FlhDC and the σS antagonist FliZ
Date
2014-08Author
Mitra, Avishek
Fay, Pamela A.
Vendura, Khoury W.
Alla, Zimrisha
Carroll, Ronan K.
Shaw, Lindsey N.
Riordan, James T.
Metadata
Show full item recordAbstract
In enterohemorrhagic Escherichia coli (EHEC), sigma factor N (σᴺ) regulates glutamate-dependent acid resistance (GDAR) and the locus of enterocyte effacement (LEE); discrete genetic systems that are required for transmission and virulence of this intestinal pathogen. Regulation of these systems requires nitrogen regulatory protein C, NtrC, and is a consequence of NtrC-σᴺ-dependent reduction in the activity of sigma factor S (σS). This study elucidates pathway components and stimuli for σᴺ-directed regulation of GDAR and the LEE in EHEC. Deletion of fliZ, the product of which reduces σS activity, phenocopied rpoN (σᴺ) and ntrC null strains for GDAR and LEE control, acid resistance, and adherence. Upregulation of fliZ by NtrC-σᴺ was shown to be indirect and required an intact flagellar regulator flhDC. Activation of flhDC by NtrC-σᴺ and FlhDC-dependent regulation of GDAR and the LEE was dependent on σᴺ-promoter flhDₚ₂, and a newly described NtrC upstream activator sequence. Addition of ammonium chloride significantly altered expression of GDAR and LEE, acid resistance, and adherence, independently of rpoN, ntrC, and the NtrC sensor kinase, ntrB. Altering the availability of NtrC phosphodonor acetyl phosphate by growth without glucose, with acetate addition, or by deletion of acetate kinase ackA, abrogated NtrC-σᴺ-dependent control of flhDC, fliZ, GDAR, and the LEE.
DOI
10.1002/mbo3.183Citation
Mitra, A., Fay, P. A., Vendura, K. W., Alla, Z., Carroll, R. K., Shaw, L. N., Riordan, J. T. (2014). σᴺ-dependent control of acid resistance and the locus of enterocyte effacement in enterohemorrhagic Escherichia coli is activated by acetyl phosphate in a manner requiring flagellar regulator FlhDC and the σS antagonist FliZ. MicrobiologyOpen, 3(4), 497-512. https://doi.org/10.1002/mbo3.183