| dc.contributor.author | Fletcher, Jacqueline | |
| dc.date.accessioned | 2018-08-17T16:24:46Z | |
| dc.date.available | 2018-08-17T16:24:46Z | |
| dc.date.issued | 1987-01 | |
| dc.identifier | oksd_jacquelinefletcher_filterpaperdot-_1987 | |
| dc.identifier.citation | Fletcher, J. (1987). Filter paper dot-immunobinding assay for detection of Spiroplasma citri. Applied and Environmental Microbiology, 53(1), 183-184. https://doi.org/10.1128/aem.53.1.183-184.1987 | |
| dc.identifier.uri | https://hdl.handle.net/11244/301426 | |
| dc.description.abstract | A rapid filter paper dot-immunobinding assay was adapted to detect the wall-less mollicute Spiroplasma citri in medium, plants, or insects. Filter paper spotted with sample was incubated first in dilute antiserum, then in protein A-peroxidase, and finally in a substrate of 4-chloro-1-naphthol plus hydrogen peroxide. The detection limit averaged 2.3 x 1010 CFU/ml in cultures, and S. citri was detected in single infected leafhoppers. This assay was less sensitive but more rapid and economical than an enzyme-linked immunosorbent assay. | |
| dc.format | application/pdf | |
| dc.language | en_US | |
| dc.publisher | American Society for Microbiology | |
| dc.rights | This material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information. | |
| dc.title | Filter paper dot-immunobinding assay for detection of Spiroplasma citri | |
| osu.filename | oksd_jacquelinefletcher_filterpaperdot-_1987.pdf | |
| dc.description.peerreview | Peer reviewed | |
| dc.identifier.doi | 10.1128/aem.53.1.183-184.1987 | |
| dc.description.department | Plant Pathology | |
| dc.type.genre | Article | |
| dc.type.material | Text | |
| dc.subject.keywords | immunological method | |
| dc.subject.keywords | rapid technique | |
| dc.subject.keywords | homoptera | |
| dc.subject.keywords | cicadellidae | |
| dc.subject.keywords | culture medium | |
| dc.subject.keywords | phytopathogen | |
| dc.subject.keywords | insecta | |
| dc.subject.keywords | arthropoda | |
| dc.subject.keywords | invertebrata | |
| dc.subject.keywords | spiroplasmataceae | |
| dc.subject.keywords | mycoplasmatales | |
