Filter paper dot-immunobinding assay for detection of Spiroplasma citri
Abstract
A rapid filter paper dot-immunobinding assay was adapted to detect the wall-less mollicute Spiroplasma citri in medium, plants, or insects. Filter paper spotted with sample was incubated first in dilute antiserum, then in protein A-peroxidase, and finally in a substrate of 4-chloro-1-naphthol plus hydrogen peroxide. The detection limit averaged 2.3 x 1010 CFU/ml in cultures, and S. citri was detected in single infected leafhoppers. This assay was less sensitive but more rapid and economical than an enzyme-linked immunosorbent assay.
Citation
Fletcher, J. (1987). Filter paper dot-immunobinding assay for detection of Spiroplasma citri. Applied and Environmental Microbiology, 53(1), 183-184. https://doi.org/10.1128/aem.53.1.183-184.1987