dc.contributor.author | Durham, Janet A. | |
dc.contributor.author | Antone, Susan M. | |
dc.contributor.author | Cunningham, Madeleine W. | |
dc.contributor.author | Confer, Anthony W. | |
dc.date.accessioned | 2015-10-16T20:47:57Z | |
dc.date.available | 2015-10-16T20:47:57Z | |
dc.date.issued | 1988-05 | |
dc.identifier | okds_Confer_JCM_1988-05.pdf | |
dc.identifier.citation | Durham, J. A., Antone, S. M., Cunningham, M. W., & Confer, A. W. (1988). Monoclonal antibodies to Pasteurella haemolytica serotype 1 lipopolysaccharide: Demonstration of antigenic similarities among several serotypes. Journal of Clinical Microbiology, 26(5), 885-889. https://doi.org/10.1128/jcm.26.5.885-889.1988 | |
dc.identifier.uri | https://hdl.handle.net/11244/19822 | |
dc.description.abstract | Murine monoclonal antibodies (MAbs) were produced which were specific for Pasteurella haemolytica serotype 1 lipopolysaccharide (LPS). The MAbs also reacted with LPS present in a partially purified antigen derived from a saline extract of the organism. The epitope to which the MAbs were directed was a carbohydrate which was sensitive to oxidation with periodate, had a molecular weight between 14,000 and 25,000 as determined by immunoblotting, and was present in a crude 0-antigen preparation of P. haemolytica LPS. The MAbs did not react with purified capsular polysaccharide from P. haemolytica serotype 1. In an enzyme-linked immunosorbent assay, reaction of the MAbs with LPS obtained from 14 gram-negative bacteria failed to detect any cross-reactivity with P. haemolytica LPS. However, the MAbs detected antigenic similarities among P. haemolytica serotypes 1, 5, 6, 7, 8, and 12 and, to a lesser extent, 4 and 14. These studies indicate that the LPS-O-antigens from several P. haemolytica serotypes have similar epitopes and may be partially responsible for shared antigenicity among serotypes. | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.publisher | American Society for Microbiology | |
dc.rights | This material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information. | |
dc.title | Monoclonal antibodies to Pasteurella haemolytica serotype 1 lipopolysaccharide: Demonstration of antigenic similarities among several serotypes | |
osu.filename | okds_Confer_JCM_1988-05.pdf | |
dc.description.peerreview | Not peer reviewed | |
dc.identifier.doi | 10.1128/jcm.26.5.885-889.1988 | |
dc.description.department | Veterinary Parasitology, Microbiology and Public Health | |
dc.description.department | Veterinary Pathology | |
dc.type.genre | Article | |
dc.type.material | Text | |