Monoclonal antibodies to Pasteurella haemolytica serotype 1 lipopolysaccharide: Demonstration of antigenic similarities among several serotypes
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Date
1988-05Author
Durham, Janet A.
Antone, Susan M.
Cunningham, Madeleine W.
Confer, Anthony W.
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Murine monoclonal antibodies (MAbs) were produced which were specific for Pasteurella haemolytica serotype 1 lipopolysaccharide (LPS). The MAbs also reacted with LPS present in a partially purified antigen derived from a saline extract of the organism. The epitope to which the MAbs were directed was a carbohydrate which was sensitive to oxidation with periodate, had a molecular weight between 14,000 and 25,000 as determined by immunoblotting, and was present in a crude 0-antigen preparation of P. haemolytica LPS. The MAbs did not react with purified capsular polysaccharide from P. haemolytica serotype 1. In an enzyme-linked immunosorbent assay, reaction of the MAbs with LPS obtained from 14 gram-negative bacteria failed to detect any cross-reactivity with P. haemolytica LPS. However, the MAbs detected antigenic similarities among P. haemolytica serotypes 1, 5, 6, 7, 8, and 12 and, to a lesser extent, 4 and 14. These studies indicate that the LPS-O-antigens from several P. haemolytica serotypes have similar epitopes and may be partially responsible for shared antigenicity among serotypes.
Citation
Durham, J. A., Antone, S. M., Cunningham, M. W., & Confer, A. W. (1988). Monoclonal antibodies to Pasteurella haemolytica serotype 1 lipopolysaccharide: Demonstration of antigenic similarities among several serotypes. Journal of Clinical Microbiology, 26(5), 885-889. https://doi.org/10.1128/jcm.26.5.885-889.1988