dc.contributor.author | Ayalew, Sahlu | |
dc.contributor.author | Confer, Anthony W. | |
dc.contributor.author | Blackwood, Emily R. | |
dc.date.accessioned | 2015-10-16T20:47:54Z | |
dc.date.available | 2015-10-16T20:47:54Z | |
dc.date.issued | 2004-12 | |
dc.identifier | okds_Confer_IAI_2004-12.pdf | |
dc.identifier.citation | Ayalew, S., Confer, A. W., & Blackwood, E. R. (2004). Characterization of immunodominant and potentially protective epitopes of Mannheimia haemolytica serotype 1 outer membrane lipoprotein PlpE. Infection and Immunity, 72(12), 7265-7274. https://doi.org/10.1128/iai.72.12.7265-7274.2004 | |
dc.identifier.uri | https://hdl.handle.net/11244/19813 | |
dc.description.abstract | Mannheimia haemolytica serotype 1 (S1) is the most common bacterial isolate found in shipping fever pneumonia in beef cattle. Currently used vaccines against M. haemolytica do not provide complete protection against the disease. Research with M. haemolytica outer membrane proteins (OMPs) has shown that antibodies to one particular OMP from S1, PlpE, may be important in immunity. In a recently published work, members of our laboratory showed that recombinant PlpE (rPlpE) is highly immunogenic when injected subcutaneously into cattle and that the acquired immunity markedly enhanced resistance to experimental challenge (A. W. Confer, S. Ayalew, R. J. Panciera, M. Montelongo, L. C. Whitworth, and J. D. Hammer, Vaccine 21:2821-2829, 2003). The objective of this work was to identify epitopes of PlpE that are responsible for inducing the immune response. Western blot analysis of a series of rPlpE with nested deletions on both termini with bovine anti-PlpE hyperimmune sera showed that the immunodominant region is located close to the N terminus of PlpE. Fine epitope mapping, in which an array of overlapping 13-mer synthetic peptides attached to a derivatized cellulose membrane was probed with various affinity-purified anti-PlpE antibodies, identified eight highly reactive regions, of which region 2 (R2) was identified as the specific epitope. The R2 region is comprised of eight imperfect repeats of a hexapeptide (QAQNAP) and is located between residues 26 and 76. Complementmediated bactericidal activity of affinity-purified anti-PlpE bovine antibodies confirmed that antibodies directed against the R2 region are effective in killing M. haemolytica. | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.publisher | American Society for Microbiology | |
dc.rights | This material has been previously published. In the Oklahoma State University Library's institutional repository this version is made available through the open access principles and the terms of agreement/consent between the author(s) and the publisher. The permission policy on the use, reproduction or distribution of the material falls under fair use for educational, scholarship, and research purposes. Contact Digital Resources and Discovery Services at lib-dls@okstate.edu or 405-744-9161 for further information. | |
dc.title | Characterization of immunodominant and potentially protective epitopes of Mannheimia haemolytica serotype 1 outer membrane lipoprotein PlpE | |
osu.filename | okds_Confer_IAI_2004-12.pdf | |
dc.description.peerreview | Peer reviewed | |
dc.identifier.doi | 10.1128/IAI.72.12.7265-7274.2004 | |
dc.description.department | Veterinary Pathobiology | |
dc.type.genre | Article | |
dc.type.material | Text | |