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Reverse transcriptase PCR amplification of Rickettsia typhi from infected mammalian cells and insect vectors
(American Society for Microbiology, 1998-06)
We developed a reverse transcriptase PCR assay to detect expression of 120- and 17-kDa antigen genes in Rickettsia typhi. Infected Vero cell and flea RNAs were reverse transcribed by using random hexamers. The cDNA was ...
Quantitation of Plasmodium falciparum sporozoites transmitted in vitro by experimentally infected Anopheles gambiae and Anopheles stephensi
(American Society of Tropical Medicine and Hygiene, 1991)
The frequency and numbers of Plasmodium falciparum sporozoites transmitted in vitro and corresponding sporozoite loads were determined for experimentally infected Anopheles gambiae and An. stephensi. Geometric mean (GM) ...
Predicting key malaria transmission factors, biting and entomological inoculation rates, using modelled soil moisture in Kenya
(Wiley, 1998-10)
While malaria transmission varies seasonally, large inter-annual heterogeneity of malaria incidence occurs. Variability in entomological parameters, biting rates and entomological inoculation rates (EIR) have been strongly ...
Flea-borne rickettsioses: Ecologic considerations
(Centers for Disease Control and Prevention (CDC), 1997-07)
Ecologic and economic factors, as well as changes in human behavior, have resulted in the emergence of new and the reemergence of existing but forgotten infectious diseases during the past 20 years. Flea-borne disease ...
Molecular identification of Rickettsia typhi and R. felis in co-infected Ctenocephalides felis (Siphonaptera: Pulicidae)
(Oxford University Press (OUP), 1998)
Rickettsia typhi and R. felis, 2 closely related rickettsial species, often have been identified in cat fleas, Ctenocephalides felis (Bouche) from the same geographical location. However, no fleas have been found to be ...
Plasmodium falciparum: Release of circumsporozoite protein by sporozoites in the mosquito vector
(Elsevier BV, 1992)
The release of circumsporozoite (CS) protein by Plasmodium falciparum sporozoites was investigated to identify factors regulating this process within infected Anopheles gambiae mosquitoes. The potential for sporozoites to ...