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Genetic and immunologic analyses of PlpE, a lipoprotein important in complement-mediated killing of Pasteurella haemolytica serotype 1
(American Society for Microbiology, 1998-12)
Pasteurella haemolytica serotype 1 is the bacterium most commonly associated with bovine shipping fever. The presence of antibodies against P. haemolytica outer membrane proteins (OMPs) correlates statistically with ...
Demonstration of age-dependent capsular material on Pasteurella haemolytica serotype 1
(American Society for Microbiology, 1982-12)
Extracellular capsular material was demonstrated on early log-phase cells of Pasteurella haemolytica serotype 1 by the fluorescent-antibody and several capsular staining techniques. The presence of this material was shown ...
Transmembrane pore size and role of cell swelling in cytotoxicity caused by Pasteurella haemolytica leukotoxin
(American Society for Microbiology, 1989-02)
Pasteurella haemolytica A1 leukotoxin causes rapid (5 to 15 min) leakage of intracellular K+ and cell swelling and slower (15 to 60 min), Ca2+-dependent formation of large plasma membrane defects (~100 nm) and leakage of ...
Effect of repeated in vitro transfer of Pasteurella haemolytica A1 on encapsulation, leukotoxin production, and virulence
(American Society for Microbiology, 1987-01)
Pasteurella haemolytica serotype 1 was transferred daily for 128 serial passages on both unsupplemented brain heart infusion agar and the same basal medium supplemented with bovine blood, horse serum, and yeast extract. ...
Enzyme-linked immunosorbent assay for detection of serum antibodies to Pasteurella haemolytica cytotoxin (leukotoxin) in cattle
(American Society for Microbiology, 1986-08)
An enzyme-linked immunosorbent assay (ELISA) was developed for detection of bovine serum antibodies to the cytotoxin (leukotoxin) of Pasteurela haemolytica. A partially purified, cytotoxic, and immunogenic protein obtained ...
Biological properties of a canine distemper virus isolate associated with demyelinating encephalomyelitis
(American Society for Microbiology, 1975-04)
A canine distemper virus (CDV), designated R252, originally recovered from a dog with demyelinating encephalomyelitis was shown to reproduce this disease in gnotobiotic dogs with a high incidence as compared to other CDV ...
Simple visual assay for determination of Pasteurella haemolytica cytotoxin neutralizing antibody titers in cattle sera
(American Society for Microbiology, 1985-12)
A simple visual assay is described for determining the capacity of bovine serum to neutralize the cytotoxin produced by Pasteurella haemolytica serotype 1. The test was reproducible from day to day with different target ...
Purification and partial characterization of the OmpA family of proteins of Pasteurella haemolytica
(American Society for Microbiology, 1997-01)
This study was conducted to partially characterize and identify the purity of two major outer membrane proteins (OMPs) (with molecular weights of 32,000 and 35,000 [32K and 35K, respectively]) of Pasteurella haemolytica. ...
Monoclonal antibodies to Pasteurella haemolytica serotype 1 lipopolysaccharide: Demonstration of antigenic similarities among several serotypes
(American Society for Microbiology, 1988-05)
Murine monoclonal antibodies (MAbs) were produced which were specific for Pasteurella haemolytica serotype 1 lipopolysaccharide (LPS). The MAbs also reacted with LPS present in a partially purified antigen derived from a ...
Characterization of immunodominant and potentially protective epitopes of Mannheimia haemolytica serotype 1 outer membrane lipoprotein PlpE
(American Society for Microbiology, 2004-12)
Mannheimia haemolytica serotype 1 (S1) is the most common bacterial isolate found in shipping fever pneumonia in beef cattle. Currently used vaccines against M. haemolytica do not provide complete protection against the ...