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Reverse transcriptase PCR amplification of Rickettsia typhi from infected mammalian cells and insect vectors
(American Society for Microbiology, 1998-06)
We developed a reverse transcriptase PCR assay to detect expression of 120- and 17-kDa antigen genes in Rickettsia typhi. Infected Vero cell and flea RNAs were reverse transcribed by using random hexamers. The cDNA was ...
Flea-borne rickettsioses: Ecologic considerations
(Centers for Disease Control and Prevention (CDC), 1997-07)
Ecologic and economic factors, as well as changes in human behavior, have resulted in the emergence of new and the reemergence of existing but forgotten infectious diseases during the past 20 years. Flea-borne disease ...