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Demonstration of age-dependent capsular material on Pasteurella haemolytica serotype 1
(American Society for Microbiology, 1982-12)
Extracellular capsular material was demonstrated on early log-phase cells of Pasteurella haemolytica serotype 1 by the fluorescent-antibody and several capsular staining techniques. The presence of this material was shown ...
Transmembrane pore size and role of cell swelling in cytotoxicity caused by Pasteurella haemolytica leukotoxin
(American Society for Microbiology, 1989-02)
Pasteurella haemolytica A1 leukotoxin causes rapid (5 to 15 min) leakage of intracellular K+ and cell swelling and slower (15 to 60 min), Ca2+-dependent formation of large plasma membrane defects (~100 nm) and leakage of ...
Effect of repeated in vitro transfer of Pasteurella haemolytica A1 on encapsulation, leukotoxin production, and virulence
(American Society for Microbiology, 1987-01)
Pasteurella haemolytica serotype 1 was transferred daily for 128 serial passages on both unsupplemented brain heart infusion agar and the same basal medium supplemented with bovine blood, horse serum, and yeast extract. ...
Enzyme-linked immunosorbent assay for detection of serum antibodies to Pasteurella haemolytica cytotoxin (leukotoxin) in cattle
(American Society for Microbiology, 1986-08)
An enzyme-linked immunosorbent assay (ELISA) was developed for detection of bovine serum antibodies to the cytotoxin (leukotoxin) of Pasteurela haemolytica. A partially purified, cytotoxic, and immunogenic protein obtained ...
Simple visual assay for determination of Pasteurella haemolytica cytotoxin neutralizing antibody titers in cattle sera
(American Society for Microbiology, 1985-12)
A simple visual assay is described for determining the capacity of bovine serum to neutralize the cytotoxin produced by Pasteurella haemolytica serotype 1. The test was reproducible from day to day with different target ...
Monoclonal antibodies to Pasteurella haemolytica serotype 1 lipopolysaccharide: Demonstration of antigenic similarities among several serotypes
(American Society for Microbiology, 1988-05)
Murine monoclonal antibodies (MAbs) were produced which were specific for Pasteurella haemolytica serotype 1 lipopolysaccharide (LPS). The MAbs also reacted with LPS present in a partially purified antigen derived from a ...
Pasteurella haemolytica antigens associated with resistance to pneumonic pasteurellosis
(American Society for Microbiology, 1989-03)
Antigens associated with whole Pasteurella haemolytica biotype A serotype 1, a capsular arbohydrateprotein extract of the organism, and P. haemolytica leukotoxin were separated by sodium dodecyl sulfatepolyacrylamide gel ...
Comparison of TRACK XI fluorometric immunoassay system with other serologic tests for detection of serum antibody to Brucella abortus in cattle
(American Society for Microbiology, 1987-02)
The TRACK XI system (TRACK) is a commercially available fluorometric immunoassay system that has been adapted for the detection of antibodies to several antigens in various animal species. Serum antibodies to Brucella ...