Method for Simultaneously Determining in a Single Multiplex Reaction Gender of Donors and Quantities of Genomic Dna and Ratios Thereof, Presence and Extent of Dna Degradation, and Pcr Inhibition Within a Human Dna Sample

Abstract

Methods are provided for determining, in a single polymerase chain reaction (PCR) reaction, the quantity, quality, and gender of origin of DNA in a sample, and whether or not the sample contains PCR amplification inhibitors. The methods involve carrying out a single PCR multiplex reaction utilizing primer sets specific for amplifying the human amelogenin locus, an X- and/or Y-chromosome specific gene that is shorter than the amelogenin gene, and a heterologous, non-human reporter gene.