Method for Simultaneously Determining in a Single Multiplex Reaction Gender of Donors and Quantities of Genomic Dna and Ratios Thereof, Presence and Extent of Dna Degradation, and Pcr Inhibition Within a Human Dna Sample
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Date
2012-04-10Author
Allen, Robert W.
Fuller, Valerie
Board of Regents/Oklahoma State University and the A & M Colleges
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Methods are provided for determining, in a single polymerase chain reaction (PCR) reaction, the quantity, quality, and gender of origin of DNA in a sample, and whether or not the sample contains PCR amplification inhibitors. The methods involve carrying out a single PCR multiplex reaction utilizing primer sets specific for amplifying the human amelogenin locus, an X- and/or Y-chromosome specific gene that is shorter than the amelogenin gene, and a heterologous, non-human reporter gene.
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