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Role of ATP binding and hydrolysis in assembly of MacAB-TolC macrolide transporter
(2013)
In Gram-negative bacteria, intrinsic antibiotic resistance is provided by three component efflux transporters. In Escherichia coli, MacAB-TolC is the first experimentally described ATP-driven tripartite efflux pump responsible ...
BIONANOFIBER-BASED NANOSTRUCTURE FABRICATION AND APPLICATIONS
(2017-12)
Among various strategies for the synthesis and assembly of nano-scaled structures, bio-templating is an efficient method due to the unique physical and chemical properties of the template bionanomaterials, thus has experienced ...
Chemical Modeling of Unstable Intermediates in Heme-mediated NOx Biology
(2017-05-12)
This dissertation describes the preparation, characterization, and reactivity of several critical unstable intermediates of relevance to the global N-cycle. Their spectroscopic signatures and chemical reactivities were ...
Imitating Nature: The Effects of Asymmetry and Strain on Copper and Iron Complexes with Schiff Base Ligands
(2013)
Metalloenzymes are incredibly complex "machines" which catalyze reactions essential for life. Metal ions are found in more than half of all known proteins; particularly, copper and iron are abundant in active sites performing ...
PROBING THE MECHANISM OF SACCHAROPINE DEHYDROGENASE FROM Saccharomyces cerevisiae USING SITE-DIRECTED MUTAGENESIS
(2010)
Saccharopine dehydrogenase (SDH) catalyses the NAD-dependent oxidative deamination of saccharopine to give L-lysine and alpha-ketoglutarate. There are a number of conserved hydrophilic, ionizable residues in the active ...
The Effect Of Ligand Denticity In Stabilizing Actinide-Element Multiple Bond Functionalities
(2017-12)
Different synthetic approaches to the development of complexes with X-An=E and E=An=E functionalities (where An = U, Th) for use in studying the inverse trans influence (ITI) and the role f-orbitals play in early actinide ...
MOBILITY STUDY OF PROTEIN AND DYE MOLECULES WITHIN SILICA HYDROGEL, LIPOSOMES, AND BACTERIA CELLS BY FLUORESCENCE MICROSCOPY
(2010)
Fluorescence techniques are very useful for understanding the various processes in biological science. This thesis presents the applications of several fluorescence techniques for studying the mobility of proteins and ...
Mechanisms of enzymes of the a-aminoadipate pathway: Homocitrate synthase from Thermus thermophilus and saccharopine dehydrogenase from Saccharomyces cerevisiae
(2011)
The &alpha-aminoadipate (AAA) pathway for lysine biosynthesis is nearly unique to higher fungi, including human and plant pathogens and euglenoids; an exception is the thermophilic bacterium Thermus thermophilus. . ...
CUCURBIT[7]URIL MEDIATED VIOLOGEN-FLUOROPHORE DYAD FOR FLUORESCENCE OFF/ON SWITCH
(2012)
CUCURBIT[7]URIL MEDIATED VIOLOGEN-FLUOROPHORE
Resolving DNA Using A Bare Open Narrow Capillary without Sieving Matrix
(2014-08)
DNA molecules encode the hereditary information utilized in all living organisms, including humans. Separating DNA fragments is essential in biological research because it informs us how DNA molecules work and eventually ...