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Studies of Hsp90 function on signal transduction molecules in C2C12 cells and effects of novobiocin on Hsp90 conformation and function and mechanism of regulation of heme-regulated eIF2alpha kinase by gas molecules
(2004-12)
Scope and Method of Study: The purpose of this study was to investigate: the function of Hsp90 on signal transduction pathways required for C2C12 myoblast differentiation; to characterize the effect of the Hsp90-inhibitor ...
Structural and functional studies of electron transfer complexes
(2005-12)
Scope and Method of Study: As an effort to understand the structure-function relationship of the electron transfer complexes, we chose Escherichia coli and Rhodobacter sphaeroides to study NADH:ubiquinone oxidoreductase ...
Studies of extra fragments of the cytochrome bc1 complex from Rhodobacter sphaeroides and the interaction between cytochrome caa3 and F1F0-ATP synthase from alkaliphilic Bacillus pseudofirmus OF4
(2006-05)
Scope and Method of Study: Although bacterial enzymes have simpler subunit composition than their mammalian counter parts, the sizes of core subunits (cytochrome b, ISP and cytochrome c1) are generally larger. Are these ...
Studies on the catalytic mechanism of cytochrome bc1 complex
(2008-07)
Scope and Method of Study: The purpose of this study is to further investigate the catalytic mechanism of cytochrome bc1 complexes. With site-directed mutagenesis, we constructed a series of mutant cytochrome bc1 complexes, ...
Studies of the cytochrome bc1 complex from Rhodobacter sphaeroides
(2008-07)
Scope and Method of Study: Determine the structure of Rhodobacter sphaeroides cytochrome bc1 complex using the engineered Super Reductase double mutant and studying the interaction between cytochrome c1 head domain and its ...
Characterization of functional domains of Saccharomyces cerevisiae septins
(2005-05)
S. cerevisiae expresses 5 septins (Cdc3p, Cdc10p, Cdc11p, Cdc12p and Shs1p) during mitotic growth. Septins interact with each other, colocalize to the cortex at the mother-bud neck, and are required for the localization ...
Characterization and functional analysis of extensins and LRR-extensin in cotton
(2004-12)
Scope and Method of Study: This research involved the characterization and functional analysis of two extensins (extensins # 2 and # 3), and an LRR-extensin (# 65631). 5' RACE experiment was done to determine the 5' ends ...