Browsing by Subject "DNA, Complementary"
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Reverse transcriptase PCR amplification of Rickettsia typhi from infected mammalian cells and insect vectors
(American Society for Microbiology, 1998-06)We developed a reverse transcriptase PCR assay to detect expression of 120- and 17-kDa antigen genes in Rickettsia typhi. Infected Vero cell and flea RNAs were reverse transcribed by using random hexamers. The cDNA was ...