Browsing by Author "Miller, Robert V."
Now showing items 1-20 of 41
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Analysis of the Coxiella burnetii type IV secretion system region I during infection
Morgan, John Kent (2009-12)Scope and Method of Study: Scope - To determine the temporal regulation of Coxiella burnetii type IV secretion system Region I homologs during infection of host cells over a time course of infection. Methods for microbiological ... -
Application of DNA probes to analysis of bacteriophage distribution patterns in the environment
Ogunseitan, O. A.; Sayler, G. S.; Miller, Robert V. (American Society for Microbiology, 1992-06)Radiolabeled bacteriophage DNA probes have been used in this study to determine the distribution of Pseudomonas aeruginosa-infecting bacteriophages in natural samples of lake water, sediment, soil, and sewage. The sensitivity ... -
Bacterial communities associated with natural and anthropogenic petroleum impacted anaerobic environments
Davis, James Paul (2012-12)Scope and Method of Study: The aim of this dissertation was to determine the diversity, abundance, distribution, and morphology of candidate division SR1 from various habitats (including a natural gas impacted fresh water ... -
Biodegradation of Petroleum Hydrocarbons by Halophilic and Halotolerant Microorganisms
Nicholson, Carla Annette (Oklahoma State University, 2005-12-01)Halophilic and halotolerant organisms can degrade pollutants, although little is known of their capacity to degrade benzene, toluene, ethylbenzene, and xylenes (BTEX). These compounds are of concern because they are stable, ... -
Characterization of Pseudomonas aeruginosa mutants deficient in the establishment of lysogeny
Miller, Robert V.; Ku, Chao-Min C. (American Society for Microbiology, 1978-06)Mutants of Pseudomonas aeruginosa with impaired ability to establish a lysogenic relationship with temperate bacteriophage (Les-) have been isolated. These les mutations map to two areas of the P. aeruginosa chromosomal ... -
Characterization of the Pseudomonas aeruginosa recA analog and its protein product: rec-102 is a mutant allele of the P. aeruginosa PAO recA gene
Kokjohn, Tyler A.; Miller, Robert V. (American Society for Microbiology, 1987-04)We cloned a 2.3-kilobase-pair fragment of the Pseudomonas aeruginosa PAO chromosome which is capable of complementing recA mutations of Escherichia coli. The recA-complementing activity was further localized to a ... -
Characterization of the Pseudomonas aeruginosa recA gene: The Les- phenotype
Kokjohn, Tyler A.; Miller, Robert V. (American Society for Microbiology, 1988-02)The Les- phenotype (lysogeny establishment deficient) is a pleiotropic effect of the lesB908 mutation of Pseudomonas aeruginosa PAO. lesB908-containing strains are also (i) deficient in general recombination, (ii) sensitive ... -
Cloning and characterization of the cl repressor of Pseudomonas aeruginosa bacteriophage D3: A functional analog of phage lambda cI protein
Miller, Robert V.; Kokjohn, Tyler A. (American Society for Microbiology, 1987-05)We cloned the gene (cl) which encodes the repressor of vegetative function of Pseudomonas aeruginosa bacteriophage D3. The cloned gene was shown to inhibit plating of D3 and the induction of D3 lysogens by UV irradiation. ... -
Conjugal transfer of R68.45 and FP5 between Pseudomonas aeruginosa strains in a freshwater environment
O'Morchoe, Susan B.; Ogunseitan, O.; Sayler, Gary S.; Miller, Robert V. (American Society for Microbiology, 1988-08)Recent concern over the release of genetically engineered organisms has resulted in a need for information about the potential for gene transfer in the environment. In this study, the conjugal transfer in Pseudomonas ... -
Continuous culture model to examine factors that affect transduction among Pseudomonas aeruginosa strains in freshwater environments
Replicon, Jean; Frankfater, Allen; Miller, Robert V. (American Society for Microbiology, 1995-09)Transduction among Pseudomonas aeruginosa strains was observed in continuous cultures operated under environmentally relevant generation times, cell densities, and phage-to-bacterium ratios, suggesting its importance as a ... -
Defining the Photosystem II Repair Regulon in Synechocystis sp.PCC6803
Nambudiri, Rekha (Oklahoma State University, 2006-07-01)Light is the primary source of photosynthesis but one of draw backs of high light is the formation of reactive oxygen species. This potentially damages the D1 protein, this proteins forms the core of PSII. DI protein is ... -
Development and characterization of a recA-based biosensor for DNA-damaging agents
Elasri, Mohamed Omar (1998-12) -
Effects of norfloxacin on DNA metabolism in Pseudomonas aeruginosa
Benbrook, Doris M.; Miller, Robert V. (American Society for Microbiology, 1986-01)Norfloxacin is a quinolone (pyridonecarboxylic acid derivative) effective against Pseudomonas aeruginosa infections. We studied the effects of this drug on DNA metabolism in P. aeruginosa. Norfloxacin inhibits DNA replication ... -
Effects of suspended particulates on the frequency of transduction among Pseudomonas aeruginosa in a freshwater environment
Ripp, Steven; Miller, Robert V. (American Society for Microbiology, 1995-04)Transduction has been shown to play a significant role in the transfer of plasmid and chromosomal DNA in aquatic ecosystems. Such ecosystems contain a multitude of environmental factors, any one of which may influence the ... -
Evidence for phage-mediated gene transfer among Pseudomonas aeruginosa strains on the phylloplane
Kidambi, Saranga P.; Ripp, Steven; Miller, Robert V. (American Society for Microbiology, 1994-02)As the use of genetically engineered microorganisms for agricultural tasks becomes more frequent, the ability of bacteria to exchange genetic material in the agricultural setting must be assessed. Transduction (bacterial ... -
Evolution of copper and streptomycin resistance in Pseudomonas syringae
Sundin, George W. (1994-05) -
Expression of the recA gene of Pseudomonas aeruginosa PAO is inducible by DNA-damaging agents
Miller, Robert V.; Kokjohn, Tyler A. (American Society for Microbiology, 1988-05)Western (immunoblot) analysis using Escherichia coli anti-RecA antiserum revealed that expression of the RecA protein of Pseudomonas aeruginosa PAO is induced upon exposure of the bacterium to UV irradiation or norfloxacin, ... -
Frequency of F116-mediated transduction of Pseudomonas aeruginosa in a freshwater environment
Morrison, W. D.; Miller, Robert V.; Sayler, G. S. (American Society for Microbiology, 1978-11)Transduction of Pseudomonas aeruginosa streptomycin resistance by a generalized transducing phage, F116, was shown to occur during a 10-day incubation in a flow-through environmental test chamber suspended in a freshwater ...