Characterization of Pseudomonas aeruginosa mutants deficient in the establishment of lysogeny
Abstract
Mutants of Pseudomonas aeruginosa with impaired ability to establish a lysogenic relationship with temperate bacteriophage (Les-) have been isolated. These les mutations map to two areas of the P. aeruginosa chromosomal map as determined by conjugational and transductional analyses. Two phenotypic classes of Les- mutants were identified. One class of mutations has pleiotropic effects on DNA metabolism. These mutants are unable to recombine genetic material acquired as a result of either conjugation or transduction (Rec-). In addition, the ability of these Les- Rec- mutants to repair UV-induced damage to bacteriophage is reduced (host-cell reactivation deficient, Hcr-). Mutants of the second class are Les-, Rec+, and Hcr+.
Citation
Miller, R. V., & Ku, C.-M. C. (1978). Characterization of Pseudomonas aeruginosa mutants deficient in the establishment of lysogeny. Journal of Bacteriology, 134(3), 875-883. https://doi.org/10.1128/jb.134.3.875-883.1978