OU - Faculty and Staff Publications

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Open Access
Ancient DNA Analysis and Stable Isotope Ecology of Sea Turtles (Cheloniidae) from the Gold Rush-era (1850s) Eastern Pacific Ocean
(2018-05-30) Kemp, Brian; Pagès Barceló, Laura; Seminoff, Jeffrey; Turner Tomaszewicz, Calandra; Labonte, Marie; Jones, Emily Lena; Stolyka, Michael; Bruner, Kale; Pastron, Allen; Conrad, Cyler
Historical and archaeological evidence documents the importation of sea turtles from the eastern Pacific Ocean (Baja California) to California during the Gold Rush (1848–1855) and through the end of 19th century, but it is unknown whether these 19th century sea turtles foraged in similar ways to their modern counterparts. To identify the species of two Gold Rush-era sea turtle specimens recovered from archaeological deposits in San Francisco, California, we first analyze ancient DNA (aDNA). We then analyze carbon (δ13Ccol), nitrogen (δ15N), and hydrogen (δD) stable isotopes of bone collagen and carbon (δ13Cap) and oxygen (δ18Oap) stable isotopes of bone apatite to test if eastern Pacific sea turtle diets have changed over the past 160 years. Ancient DNA confirms that both archaeological specimens are green sea turtles (Chelonia mydas). The stable isotope values from the 19th-century specimens are statistically indistinguishable from the modern comparatives in both δ13Ccoland δ15N, suggesting that green sea turtle dietary intake has remained relatively unchanged since the 1850s. However, the values are unclear for δD and δ18Oapand require additional research.
Open Access
Biogeographic problem-solving reveals the Late Pleistocene translocation of a short-faced bear to the California Channel Islands
(2020-09-16) Mychajliw, Alexis M.; Rick, Torben C.; Dagtas, Nihan D.; Erlandson, Jon M.; Culleton, Brendan J.; Kennett, Douglas J.; Buckley, Michael; Hofman, Courtney A.
An accurate understanding of biodiversity of the past is critical for contextualizing biodiversity patterns and trends in the present. Emerging techniques are refining our ability to decipher otherwise cryptic human-mediated species translocations across the Quaternary, yet these techniques are often used in isolation, rather than part of an interdisciplinary hypothesis-testing toolkit, limiting their scope and application. Here we illustrate the use of such an integrative approach and report the occurrence of North America’s largest terrestrial mammalian carnivore, the short-faced bear, Arctodus simus, from Daisy Cave (CA-SMI-261), an important early human occupation site on the California Channel Islands. We identified the specimen by corroborating morphological, protein, and mitogenomic lines of evidence, and evaluated the potential natural and anthropogenic mechanisms of its transport and deposition. While representing just a single specimen, our combination of techniques opened a window into the behavior of an enigmatic species, suggesting that A. simus was a wide-ranging scavenger utilizing terrestrial and marine carcasses. This discovery highlights the utility of bridging archaeological and paleontological datasets to disentangle complex biogeographic scenarios and reveal unexpected biodiversity for island systems worldwide.
Open Access
Biogeographic Study of Human Gut-Associated CrAssphage Suggests Impacts From Industrialization and Recent Expansion
(2020-01-15) Honap, Tanvi P.; Sankaranarayanan, Krithivasan; Schnorr, Stephanie L.; Ozga, Andrew T.; Warinner, Christina; Lewis Jr., Cecil M.
CrAssphage (cross-assembly phage) is a bacteriophage that was first discovered in human gut metagenomic data. CrAssphage belongs to a diverse family of crAss-like bacteriophages thought to infect gut commensal bacteria belonging to Bacteroides species. However, not much is known about the biogeography of crAssphage and whether certain strains are associated with specific human populations. In this study, we screened publicly available human gut metagenomic data from 3,341 samples for the presence of crAssphage sensu stricto (NC_024711.1). We found that crAssphage prevalence is low in traditional, hunter-gatherer populations, such as the Hadza from Tanzania and Matses from Peru, as compared to industrialized, urban populations. Statistical comparisons showed no association of crAssphage prevalence with variables such as age, sex, body mass index, and health status of individuals. Phylogenetic analyses show that crAssphage strains reconstructed from the same individual over multiple time-points, cluster together. CrAssphage strains from individuals from the same study population do not always cluster together. Some evidence of clustering is seen at the level of broadly defined geographic regions, however, the relative positions of these clusters within the crAssphage phylogeny are not well-supported. We hypothesize that this lack of strong biogeographic structuring is suggestive of an expansion event within crAssphage. Using a Bayesian dating approach, we estimate that this expansion has occurred fairly recently. Overall, we determine that crAssphage presence is associated with an industrialized lifestyle and the absence of strong biogeographic structuring within global crAssphage strains is likely due to a recent population expansion within this bacteriophage.
Open Access
Collagen Fingerprinting and the Earliest Marine Mammal Hunting in North America
(2018-07-03) Hofman, Courtney A.; Rick, Torben C.; Erlandson, Jon M.; Reeder-Myers, Leslie; Welch, Andreanna J.; Buckley, Michael
The submersion of Late Pleistocene shorelines and poor organic preservation at many early archaeological sites obscure the earliest effects of humans on coastal resources in the Americas. We used collagen fingerprinting to identify bone fragments from middens at four California Channel Island sites that are among the oldest coastal sites in the Americas (~12,500-8,500 cal BP). We document Paleocoastal human predation of at least three marine mammal families/species, including northern elephant seals (Mirounga angustirostris), eared seals (Otariidae), and sea otters (Enhydra lutris). Otariids and elephant seals are abundant today along the Pacific Coast of North America, but elephant seals are rare in late Holocene (<1500 cal BP) archaeological sites. Our data support the hypotheses that: (1) marine mammals helped fuel the peopling of the Americas; (2) humans affected marine mammal biogeography millennia before the devastation caused by the historic fur and oil trade; and (3) the current abundance and distribution of recovering pinniped populations on the California Channel Islands may mirror a pre-human baseline.
Open Access
Do I have something in my teeth? The trouble with genetic analyses of diet from archaeological dental calculus
(2020) Mann, Allison E.; Fellows Yates, James A.; Fagernäs, Zandra; Austin, Rita M.; Nelson, Elizabeth A.; Hofman, Courtney A.
Dental calculus and other preserved microbiome substrates are an attractive target for dietary reconstruction in past populations through a variety of physical, chemical, and molecular means. Recently, studies have attempted to reconstruct diet from archaeological dental calculus using archaeogenetic techniques. While dental calculus may provide a relatively stable environment for DNA preservation, the detection of plants and animals possibly consumed by an individual through DNA analysis is primarily hindered by microbial richness and incomplete reference databases. Moreover, high genomic similarity within eukaryotic groups - such as mammals - can obfuscate precise taxonomic identification. In the current study we demonstrate the challenges associated with accurate taxonomic identification and authentication of dietary taxa in ancient DNA data using both synthetic and ancient dental calculus datasets. We highlight common errors and sources of contamination across ancient DNA datasets, provide recommendations for dietary DNA validation, and call for caution in the interpretation of diet from dental calculus and other archaeological microbiome substrates.
Open Access
The Earliest Farmers of Northwest China Exploited Grain-fed Pheasants not Chickens
(2020-02-13) Barton, Loukas; Bingham, Brittany; Sankaranarayanan, Krithivasan; Monroe, Cara; Thomas, Ariane; Kemp, Brian M.
Though chickens (Gallus gallus domesticus) are globally ubiquitous today, the timing, location, and manner of their domestication is contentious. Until recently, archaeologists placed the origin of the domestic chicken in northern China, perhaps as early as 8,000 years ago. Such evidence however complicates our understanding of how the chicken was domesticated because its wild progenitor – the red jungle fowl (Gallus gallus) – lives in tropical ecosystems and does not exist in northern China today or in the recent past. Increasingly, multiple lines of evidence suggest that many of the archaeological bird remains underlying this northern origins hypothesis have been misidentified. Here we analyze the mitochondrial DNA of some of the earliest purported chickens from the Dadiwan site in northern China and conclude that they are pheasants (Phasianus colchicus). Curiously, stable isotope values from the same birds reveal that their diet was heavy in agricultural products (namely millet), meaning that they lived adjacent to or among some of the earliest farming communities in East Asia. We suggest that the exploitation of these baited birds was an important adaptation for early farmers in China’s arid north, and that management practices like these likely played a role in the domestication of animals – including the chicken – in similar contexts throughout the region.
Open Access
Ethical Priority of the Most Actionable System of Biomolecules: the Metabolome
(2019-10-23) Lewis, Cecil M.; McCall, Laura-Isobel; Sharp, Richard R.; Spicer, Paul G.
The metabolome is a system of small biomolecules (metabolites) and a direct result of human bioculture. Consequently, metabolomics is well poised to impact anthropological and biomedical research for the foreseeable future. Overall, we provide a perspective on the ethical, legal, and social implications (ELSI) of metabolomics, which we argue are often more alarming than those of genomics. Given the current mechanisms to fund research, ELSI beyond human DNA is stifled and in need of considerable attention.
Open Access
How Jeffries Wyman put Florida and Shell Mounds on the Map
(2015-05-27) Randall, Asa
The state of Florida is an intermittent flashpoint of shell mound research. This process was initiated in the 19th century by Jeffries Wyman, whose 1875 publication Fresh-Water Shell Mounds of the St. John’s River, Florida highlighted methods that are now commonplace. Wyman’s innovations are often attributed to familiarity with the Scandinavian shell midden school. I examine how Wyman’s experiences engaging with the archaeology of northeast Florida also structured his methods and interpretations. I employ GIS to reconstruct Wyman’s reconnaissance surveys based on his daily field journals. I consider the characteristics of his encampments and changing field methods. By visualizing Wyman’s excursions, I consider why he emphasized certain sites and observations over others, and reconstruct how he conducted his excavations. The results have significance for local researchers who use Wyman’s research, and anyone interested in the influence of field experiences on the emergence of prehistory as scientific inquiry and its theoretical consequences.
Open Access
Increased mitochondrial DNA diversity in ancient Columbia River basin Chinook salmon Oncorhynchus tshawytscha
(2018-01-10) Johnson, Bobbi M.; Kemp, Brian M.; Thorgaard, Gary; University of Oklahoma Libraries Open Access Fund
The Columbia River and its tributaries provide essential spawning and rearing habitat for many salmonid species, including Chinook salmon (Oncorhynchus tshawytscha). Chinook salmon were historically abundant throughout the basin and Native Americans in the region relied heavily on these fish for thousands of years. Following the arrival of Europeans in the 1800s, salmon in the basin experienced broad declines linked to overfishing, water diversion projects, habitat destruction, connectivity reduction, introgression with hatchery-origin fish, and hydropower development. Despite historical abundance, many native salmonids are now at risk of extinction. Research and management related to Chinook salmon is usually explored under what are termed “the four H’s”: habitat, harvest, hatcheries, and hydropower; here we explore a fifth H, history. Patterns of prehistoric and contemporary mitochondrial DNA variation from Chinook salmon were analyzed to characterize and compare population genetic diversity prior to recent alterations and, thus, elucidate a deeper history for this species. A total of 346 ancient and 366 contemporary samples were processed during this study. Species was determined for 130 of the ancient samples and control region haplotypes of 84 of these were sequenced. Diversity estimates from these 84 ancient Chinook salmon were compared to 379 contemporary samples. Our analysis provides the first direct measure of reduced genetic diversity for Chinook salmon from the ancient to the contemporary period, as measured both in direct loss of mitochondrial haplotypes and reductions in haplotype and nucleotide diversity. However, these losses do not appear equal across the basin, with higher losses of diversity in the mid-Columbia than in the Snake subbasin. The results are unexpected, as the two groups were predicted to share a common history as parts of the larger Columbia River Basin, and instead indicate that Chinook salmon in these subbasins may have divergent demographic histories.
Open Access
Intrinsic challenges in ancient microbiome reconstruction using 16S rRNA gene amplification
(2015-11-13) Warriner, Christina; Lewis, Cecil; Mann, Allison; Sankaranarayanan, Krithivasan; Ozga, Andrew
To date, characterization of ancient oral (dental calculus) and gut (coprolite) microbiota has been primarily accomplished through a metataxonomic approach involving targeted amplification of one or more variable regions in the 16S rRNA gene. Specifically, the V3 region (E. coli 341–534) of this gene has been suggested as an excellent candidate for ancient DNA amplification and microbial community reconstruction. However, in practice this metataxonomic approach often produces highly skewed taxonomic frequency data. In this study, we use non-targeted (shotgun metagenomics) sequencing methods to better understand skewed microbial profiles observed in four ancient dental calculus specimens previously analyzed by amplicon sequencing. Through comparisons of microbial taxonomic counts from paired amplicon (V3 U341F/534R) and shotgun sequencing datasets, we demonstrate that extensive length polymorphisms in the V3 region are a consistent and major cause of differential amplification leading to taxonomic bias in ancient microbiome reconstructions based on amplicon sequencing. We conclude that systematic amplification bias confounds attempts to accurately reconstruct microbiome taxonomic profiles from 16S rRNA V3 amplicon data generated using universal primers. Because in silico analysis indicates that alternative 16S rRNA hypervariable regions will present similar challenges, we advocate for the use of a shotgun metagenomics approach in ancient microbiome reconstructions.