An Exploration of the Potential Role of the Synthesis Between Low-density Lipoproteins and Reduced Graphene Oxide in Laser Immunotherapy Cancer Treatment

Abstract

Laser immunotherapy (LIT) is a cancer treatment modality which utilizes a photosensitizer and an immunoadjuvant to spark an immune response after lasing the primary tumor, which then allows for the destruction of metastases around the body. It is possible that the materials on which LIT relies could be transported to cancerous cells via the use of low-density lipoproteins (LDL), which occur naturally in mammals. This delivery method makes use of the overexpression of LDL receptors (LDLR) by cancer lines. This increased uptake will be confirmed via western blot. Previously documented effects of reduced graphene oxide (rGO) suggest its useful nature in LIT, rendering it a prime candidate for integration into an LDL nanoparticle. This study will perform assays on a synthesis between these two materials, dubbed rGO-LDL, to assess its impact in cells and under laser conditions. Tests will include an investigation of rGO-LDL uptake in healthy and infected cells, toxicity, and migration inhibition, repeated both with and without laser treatment.