A YoeB toxin cleaves both RNA and DNA

McGillick, Julia; Ames, Jessica R.; Murphy, Tamiko; Bourne, Christina R.

A YoeB toxin cleaves both RNA and DNA

dc.contributor.author	McGillick, Julia
dc.contributor.author	Ames, Jessica R.
dc.contributor.author	Murphy, Tamiko
dc.contributor.author	Bourne, Christina R.
dc.date.accessioned	2021-05-26T14:34:17Z
dc.date.available	2021-05-26T14:34:17Z
dc.date.issued	2021-02-11
dc.description.abstract	Type II toxin-antitoxin systems contain a toxin protein, which mediates diverse interactions within the bacterial cell when it is not bound by its cognate antitoxin protein. These toxins provide a rich source of evolutionarily-conserved tertiary folds that mediate diverse catalytic reactions. These properties make toxins of interest in biotechnology applications, and studies of the catalytic mechanisms continue to provide surprises. In the current work, our studies on a YoeB family toxin from Agrobacterium tumefaciens have revealed a conserved ribosome-independent non-specific nuclease activity. We have quantified the RNA and DNA cleavage activity, revealing they have essentially equivalent dose-dependence while differing in requirements for divalent cations and pH sensitivity. The DNA cleavage activity is as a nickase for any topology of double-stranded DNA, as well as cleaving single-stranded DNA. AtYoeB is able to bind to double-stranded DNA with mid-micromolar affinity. Comparison of the ribosome-dependent and -independent reactions demonstrates an approximate tenfold efficiency imparted by the ribosome. This demonstrates YoeB toxins can act as non-specific nucleases, cleaving both RNA and DNA, in the absence of being bound within the ribosome.	en_US
dc.description.peerreview	Yes	en_US
dc.description.sponsorship	This work was supported by an Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health [P20GM103640]. Financial support was provided from the Office of the Vice President for Research and the Office of the Provost, University of Oklahoma. Open Access fees paid for in whole or in part by the University of Oklahoma Libraries.	en_US
dc.identifier.citation	McGillick, J., Ames, J.R., Murphy, T. et al. A YoeB toxin cleaves both RNA and DNA. Sci Rep 11, 3592 (2021). https://doi.org/10.1038/s41598-021-82950-6	en_US
dc.identifier.doi	10.1038/s41598-021-82950-6	en_US
dc.identifier.uri	https://hdl.handle.net/11244/330039
dc.language	en_US	en_US
dc.rights	Attribution 4.0 International	*
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	*
dc.subject	Applied Microbiology	en_US
dc.subject	Nucleases	en_US
dc.title	A YoeB toxin cleaves both RNA and DNA	en_US
dc.type	Article	en_US
ou.group	College of Arts and Sciences::Department of Chemistry and Biochemistry	en_US