Antibacterial Properties of MgO Nanoparticles Immobilized Polycaprolactone to Staphylococcus Aureus

Abstract

Titanium-based implants have been widely used in orthopaedics and orthodontic surgeries because of their strong mechanical, chemical and biological properties. We have invented a set of steps (e.g. grooving and oxidizing) by which a nanofiber matrix (NFM), composed of collagen (CG) and poly-?-caprolactone (PCL) electrospun nanofibers, can be coated on a Ti implant without subsequent detachment. A significantly improved osseointegration of CG-PCL NFM-coated Ti over non-coated Ti was observed in our experiments. MgO NP shows promising antimicrobial properties with minimal toxicity1 and excellent biocompatibility with osteoblast cells in CG-PCL NFM and poly-methyl-methacrylate (PMMA) bone cement.2 Prolonged anti-bacterial activities of an implant are possible by tethering anti-bacterial molecules with the implant by microgrooving the implant and subsequently coating the implant with MgO NP tethered PCL NFM.3 The effect of MgO NP tethered PCL on the antimicrobial activities of PCL-NFM is not known, which then leads to this study. The goal of this study is to evaluate the antimicrobial properties of PCL without and with different concentration of MgO nanoparticles using Staphylococcus aureus (ATCC 6538).