Perspectives on Viral RNA Genomes and the RNA Folding Problem

Schroeder, Susan J.

Perspectives on Viral RNA Genomes and the RNA Folding Problem

dc.contributor.author	Schroeder, Susan J.
dc.date.accessioned	2021-02-03T20:57:07Z
dc.date.available	2021-02-03T20:57:07Z
dc.date.issued	2020-10-05
dc.description.abstract	Viral RNA genomes change shape as virus particles disassemble, form replication complexes, attach to ribosomes for translation, evade host defense mechanisms, and assemble new virus particles. These structurally dynamic RNA shapeshifters present a challenging RNA folding problem, because the RNA sequence adopts multiple structures and may sometimes contain regions of partial disorder. Recent advances in high resolution asymmetric cryoelectron microscopy and chemical probing provide new ways to probe the degree of structure and disorder, and have identified more than one conformation in dynamic equilibrium in viral RNA. Chemical probing and the Detection of RNA Folding Ensembles using Expectation Maximization (DREEM) algorithm has been applied to studies of the dynamic equilibrium conformations in HIV RNA in vitro, in virio, and in vivo. This new type of data provides insight into important questions about virus assembly mechanisms and the fundamental physical forces driving virus particle assembly.	en_US
dc.description.peerreview	Yes	en_US
dc.description.sponsorship	This research was funded by a seed grant from the Oklahoma Health Science Center Office of the Vice President for Research and the Presbyterian Health Foundation. Open Access fees paid for in whole or in part by the University of Oklahoma Libraries.	en_US
dc.identifier.citation	Schroeder SJ. Perspectives on Viral RNA Genomes and the RNA Folding Problem. Viruses. 2020; 12(10):1126. https://doi.org/10.3390/v12101126	en_US
dc.identifier.doi	10.3390/v12101126	en_US
dc.identifier.uri	https://hdl.handle.net/11244/328586
dc.language	en_US	en_US
dc.rights	Attribution 4.0 International	*
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	*
dc.subject	Structural virology	en_US
dc.subject	Viral RNA folding	en_US
dc.subject	Encapsidated viral RNA	en_US
dc.title	Perspectives on Viral RNA Genomes and the RNA Folding Problem	en_US
dc.type	Article	en_US
ou.group	College of Arts and Sciences::Department of Chemistry and Biochemistry	en_US