Characterization and incorporation of Mycobacteriophage Fulbright into a polycaprolactone nanofiber wound dressing

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dc.contributor.advisorKotturi, Hari
dc.contributor.authorLopez-Davis, Charmaine M.
dc.contributor.committeeMemberMorshed, Khandaker
dc.contributor.committeeMemberOlson, Paul
dc.date.accessioned2022-08-24T19:32:11Z
dc.date.available2022-08-24T19:32:11Z
dc.date.issued2022
dc.description.abstractNontuberculous mycobacteria infections such as Mycobacterium abscessus have become a growing concern due to the emergence of multidrug-resistant bacteria, making treatment of infections difficult. Bacteriophages, or phages, are viruses that can infect bacterial cells without harming eukaryotic cells. Bacteriophage therapy is a potential alternative for treating mycobacterial skin infections as phages only kill their bacterial host, leaving the normal flora unharmed. Mycobacteriophage Fulbright was characterized and determined to possess qualities suitable for phage therapy. Fulbright remained stable at temperatures 20-60°C and pH 4-9. The replication cycle took approximately 3 hours to complete, with a 90-minute latent phase. At high titer concentrations, Fulbright was able to lyse M. abscessus, a human pathogen. Fulbright was incorporated into polycaprolactone (PCL) fibers to serve as a model antibacterial wound dressing. PCL_Fulbright effectively reduced the concentration of M. smegmatis and was observed to be non-toxic to fibroblast cells. Incorporated into the PCL fiber, the phage was stable for up to 11 months when stored at -20°C. This project is notable because it was the first mycobacteriophage incorporated into a nanofiber for phage therapy applications and serves as a foundation for future projects.en_US
dc.identifier.oclc(OCoLC)1347403100
dc.identifier.other(AlmaMMSId)9982882505002196
dc.identifier.urihttps://hdl.handle.net/11244/336502
dc.rightsAll rights reserved by the author, who has granted UCO Chambers Library the non-exclusive right to share this material in its online repositories. Contact UCO Chambers Library's Digital Initiatives Working Group at diwg@uco.edu for the permission policy on the use, reproduction or distribution of this material.
dc.subject.keywordsBacteriophage
dc.subject.keywordsMycobacteriophage
dc.subject.keywordsMycobacterium
dc.subject.keywordsNanofiber
dc.subject.keywordsPolycaprolactone
dc.subject.keywordsVirology
dc.subject.lcshBacteriophages
dc.subject.lcshTherapeutic use
dc.subject.lcshType specimens
dc.subject.lcshBandages and bandaging
dc.subject.lcshTechnological innovations
dc.subject.lcshMycobacterial diseases
dc.subject.lcshTreatment
dc.subject.lcshWounds and injuries
dc.subject.lcshAntibacterial agents
dc.thesis.degreeM.S., Biology
dc.titleCharacterization and incorporation of Mycobacteriophage Fulbright into a polycaprolactone nanofiber wound dressingen_US
dc.typeAcademic theses
thesis.degree.grantorJackson College of Graduate Studies

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