Transcriptional Regulator PerA Influences Biofilm-Associated, Platelet Binding, and Metabolic Gene Expression in Enterococcus faecalis

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dc.contributor.authorScott M. Maddoxen_US
dc.contributor.authorPhillip S. Coburnen_US
dc.contributor.authorNathan Shankaren_US
dc.contributor.authorTyrrell Conwayen_US
dc.date.accessioned2015-01-23T17:17:34Z
dc.date.accessioned2016-03-30T15:35:45Z
dc.date.available2015-01-23T17:17:34Z
dc.date.available2016-03-30T15:35:45Z
dc.date.issued2012-04-04en_US
dc.descriptionen_US
dc.descriptionConceived and designed the experiments: SMM PSC NS TC. Performed the experiments: SMM PSC. Analyzed the data: SMM PSC NS TC. Contributed reagents/materials/analysis tools: SMM PSC NS TC. Wrote the paper: SMM.en_US
dc.description.abstractEnterococcus faecalis is an opportunistic pathogen and a leading cause of nosocomial infections, traits facilitated by the ability to quickly acquire and transfer virulence determinants. A 150 kb pathogenicity island (PAI) comprised of genes contributing to virulence is found in many enterococcal isolates and is known to undergo horizontal transfer. We have shown that the PAI-encoded transcriptional regulator PerA contributes to pathogenicity in the mouse peritonitis infection model. In this study, we used whole-genome microarrays to determine the PerA regulon. The PerA regulon is extensive, as transcriptional analysis showed 151 differentially regulated genes. Our findings reveal that PerA coordinately regulates genes important for metabolism, amino acid degradation, and pathogenicity. Further transcriptional analysis revealed that PerA is influenced by bicarbonate. Additionally, PerA influences the ability of E. faecalis to bind to human platelets. Our results suggest that PerA is a global transcriptional regulator that coordinately regulates genes responsible for enterococcal pathogenicity.en_US
dc.description.peerreviewYesen_US
dc.description.peerreviewnoteshttp://www.plosone.org/static/editorial#peeren_US
dc.identifier.citationMaddox SM, Coburn PS, Shankar N, Conway T (2012) Transcriptional Regulator PerA Influences Biofilm-Associated, Platelet Binding, and Metabolic Gene Expression in Enterococcus faecalis. PLoS ONE 7(4): e34398. doi:10.1371/journal.pone.0034398en_US
dc.identifier.doi10.1371/journal.pone.0034398en_US
dc.identifier.urihttp://hdl.handle.net/11244/14073
dc.language.isoen_USen_US
dc.publisherPLos Oneen_US
dc.relation.ispartofseriesPLoS ONE 7(4):e34398en_US
dc.relation.urihttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0034398en_US
dc.rightsAttribution 3.0 United Statesen_US
dc.rights.requestablefalseen_US
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/en_US
dc.subjectPLOSen_US
dc.subjectPublic Library of Scienceen_US
dc.subjectOpen Accessen_US
dc.subjectOpen-Accessen_US
dc.subjectScienceen_US
dc.subjectMedicineen_US
dc.subjectBiologyen_US
dc.subjectResearchen_US
dc.subjectPeer-reviewen_US
dc.subjectInclusiveen_US
dc.subjectInterdisciplinaryen_US
dc.subjectAnte-disciplinaryen_US
dc.subjectPhysicsen_US
dc.subjectChemistryen_US
dc.subjectEngineeringen_US
dc.titleTranscriptional Regulator PerA Influences Biofilm-Associated, Platelet Binding, and Metabolic Gene Expression in Enterococcus faecalisen_US
dc.typeResearch Articleen_US

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