Role of Catecholate Siderophores in Gram-Negative Bacterial Colonization of the Mouse Gut

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dc.contributor.authorHualiang Pien_US
dc.contributor.authorShari A. Jonesen_US
dc.contributor.authorLynn E. Merceren_US
dc.contributor.authorJessica P. Meadoren_US
dc.contributor.authorJoyce E. Caughronen_US
dc.contributor.authorLorne Jordanen_US
dc.contributor.authorSalete M. Newtonen_US
dc.contributor.authorTyrrell Conwayen_US
dc.contributor.authorPhillip E. Klebbaen_US
dc.date.accessioned2015-01-23T17:17:41Z
dc.date.accessioned2016-03-30T15:35:39Z
dc.date.available2015-01-23T17:17:41Z
dc.date.available2016-03-30T15:35:39Z
dc.date.issued2012-11-29en_US
dc.descriptionen_US
dc.descriptionConceived and designed the experiments: PEK SMN TC. Performed the experiments: HP SAJ LEM JPM JEC LJ SMN PEK. Analyzed the data: HP SAJ LEM JPM JEC LJ SMN TC PEK. Contributed reagents/materials/analysis tools: PEK TC. Wrote the paper: SMN PEK TC.en_US
dc.description.abstractWe investigated the importance of the production of catecholate siderophores, and the utilization of their iron (III) complexes, to colonization of the mouse intestinal tract by Escherichia coli. First, a ΔtonB strain was completely unable to colonize mice. Next, we compared wild type E. coli MG1655 to its derivatives carrying site-directed mutations of genes for enterobactin synthesis (ΔentA::Cm; strain CAT0), ferric catecholate transport (Δfiu, ΔfepA, Δcir, ΔfecA::Cm; CAT4), or both (Δfiu, ΔfepA, ΔfecA, Δcir, ΔentA::Cm; CAT40) during colonization of the mouse gut. Competitions between wild type and mutant strains over a 2-week period in vivo showed impairment of all the genetically engineered bacteria relative to MG1655. CAT0, CAT4 and CAT40 colonized mice 101-, 105-, and 102-fold less efficiently, respectively, than MG1655. Unexpectedly, the additional inability of CAT40 to synthesize enterobactin resulted in a 1000-fold better colonization efficiency relative to CAT4. Analyses of gut mucus showed that CAT4 hyperexcreted enterobactin in vivo, effectively rendering the catecholate transport-deficient strain iron-starved. The results demonstrate that, contrary to prior reports, iron acquisition via catecholate siderophores plays a fundamental role in bacterial colonization of the murine intestinal tract.en_US
dc.description.peerreviewYesen_US
dc.description.peerreviewnoteshttp://www.plosone.org/static/editorial#peeren_US
dc.identifier.citationPi H, Jones SA, Mercer LE, Meador JP, Caughron JE, et al. (2012) Role of Catecholate Siderophores in Gram-Negative Bacterial Colonization of the Mouse Gut. PLoS ONE 7(11): e50020. doi:10.1371/journal.pone.0050020en_US
dc.identifier.doi10.1371/journal.pone.0050020en_US
dc.identifier.urihttp://hdl.handle.net/11244/14082
dc.language.isoen_USen_US
dc.publisherPLos Oneen_US
dc.relation.ispartofseriesPLoS ONE 7(11):e50020en_US
dc.relation.urihttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0050020en_US
dc.rightsAttribution 3.0 United Statesen_US
dc.rights.requestablefalseen_US
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/en_US
dc.subjectPLOSen_US
dc.subjectPublic Library of Scienceen_US
dc.subjectOpen Accessen_US
dc.subjectOpen-Accessen_US
dc.subjectScienceen_US
dc.subjectMedicineen_US
dc.subjectBiologyen_US
dc.subjectResearchen_US
dc.subjectPeer-reviewen_US
dc.subjectInclusiveen_US
dc.subjectInterdisciplinaryen_US
dc.subjectAnte-disciplinaryen_US
dc.subjectPhysicsen_US
dc.subjectChemistryen_US
dc.subjectEngineeringen_US
dc.titleRole of Catecholate Siderophores in Gram-Negative Bacterial Colonization of the Mouse Guten_US
dc.typeResearch Articleen_US

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