Broad-Band Activatable White-Opsin

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dc.contributor.authorSubrata Batabyal
dc.contributor.authorGregory Cervenka
dc.contributor.authorJi Hee Ha
dc.contributor.authorYoung-tae Kim
dc.contributor.authorSamarendra Mohanty
dc.date.accessioned2017-03-05T22:55:10Z
dc.date.available2017-03-05T22:55:10Z
dc.date.issued2015-09-11
dc.descriptionThe authors would like to thank C. Cote and K. Dhakal (UTA) for help during initiation of the project. SM would like to thank K. Deisseroth (Stanford University) for ChR2 and C1V1 plasmids, and J. Lin (UCSD) for the ReaChR construct.en_US
dc.descriptionen_US
dc.description.abstractCurrently, the use of optogenetic sensitization of retinal cells combined with activation/inhibition has the potential to be an alternative to retinal implants that would require electrodes inside every single neuron for high visual resolution. However, clinical translation of optogenetic activation for restoration of vision suffers from the drawback that the narrow spectral sensitivity of an opsin requires active stimulation by a blue laser or a light emitting diode with much higher intensities than ambient light. In order to allow an ambient light-based stimulation paradigm, we report the development of a ‘white-opsin’ that has broad spectral excitability in the visible spectrum. The cells sensitized with white-opsin showed excitability at an order of magnitude higher with white light compared to using only narrow-band light components. Further, cells sensitized with white-opsin produced a photocurrent that was five times higher than Channelrhodopsin-2 under similar photo-excitation conditions. The use of fast white-opsin may allow opsin-sensitized neurons in a degenerated retina to exhibit a higher sensitivity to ambient white light. This property, therefore, significantly lowers the activation threshold in contrast to conventional approaches that use intense narrow-band opsins and light to activate cellular stimulation.en_US
dc.description.peerreviewYesen_US
dc.description.peerreviewnoteshttp://www.plosone.org/static/editorial#peeren_US
dc.identifier.citationBatabyal S, Cervenka G, Ha JH, Kim Y-t, Mohanty S (2015) Broad-Band Activatable White-Opsin. PLoS ONE 10(9): e0136958. doi:10.1371/journal.pone.0136958en_US
dc.identifier.doi10.1371/journal.pone.0136958en_US
dc.identifier.urihttp://hdl.handle.net/11244/49270
dc.language.isoen_USen_US
dc.publisherPLos One
dc.relation.ispartofseriesPLoS ONE 10(9): e0136958
dc.relation.urihttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0136958
dc.rightsAttribution 3.0 United States
dc.rights.requestablefalseen_US
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/
dc.subjectLight,White light,Optogenetics,Functional electrical stimulation,Fluorescence microscopy,Visible light,Fluorescence imaging,Light pulsesen_US
dc.titleBroad-Band Activatable White-Opsinen_US
dc.typeResearch Articleen_US

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