Effect of Immobilization of Fibronectin and Collagen On the Cellular Functions of Poly-&[epsilon]-caprolactone

Abstract

Fibronectin (FN) contains several active sites, known as the heparin-binding domains, collagen-binding domain, fibrin-binding domain, and cell-binding domain, that serve as platforms for cell anchorage. [1] The goal of this study was to evaluate the effect of immobilization of collagen and plasma fibronectin with polycaprolactone (PCL) nanofiber membrane (NFM) on the cellular functions of PCL NFM.The results (Fig. 1c and Fig. 1d) show that the individual immobilization of CG and FN on PCL NFM has no adverse effect on osteoblast cell adhesion and proliferation of PCL NFM, although a significant increase of cell adhesion was observed for FN-PCL-NFM when compared to PCL NFM (p<0.05). A significant improvement of cell adhesion and proliferation was observed for FN-CG-PCL NFM in comparison to PCL NFM (p <0.01). This is due to the fact that higher cell functions were created via better cell signaling arising from the cell–cell contact and the cell-NFM components in the case of FN-CG immobilized PCL NFM compared to PCL NFM.