Validation of enzyme-linked immunosorbent assays for the detection of licit and illicit drugs in human breast milk.

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dc.contributor.advisorJourdan, Thomas
dc.contributor.authorFukuda, Tamiko H.
dc.contributor.committeeMemberBost, Robert
dc.contributor.committeeMembervon Minden, David
dc.date.accessioned2020-05-26T20:39:26Z
dc.date.available2020-05-26T20:39:26Z
dc.date.issued2012
dc.description.abstractHuman breast milk contains essential nutrients and immunological factors that are critical for the health and development of infants. The benefits of breast-feeding have been studied extensively, and research has shown that breastfed infants have a decreased risk of infections and illnesses. There are many instances when mothers are unable to provide their own milk, which is the case with many prematurely born infants. Breast milk banks and facilities that process human milk provide an alternative solution to synthetic or animal derived infant formula, allowing babies to receive the benefits of human breast milk. There are many drugs that can pass into a woman's breast milk and cause possible harm to an infant. It is important that donor milk be screened for drugs-of-abuse in order to prevent this from occurring. The purpose of this study was to optimize and validate enzyme-linked immunosorbent assays (ELISA) for the detection of a seven-drug panel in human breast milk. The following Neogen Corporation kits were utilized: Amphetamine Ultra, Benzodiazepine Group, Cocaine/Benzoylecgonine (BZE), Cotinine, Opiate Group, Oxycodone/Oxymorphone, and THC. Sample dilutions that minimized breast milk matrix interference were determined, and cutoff levels for each assay were proposed based on the linear range of the assay. The seven-drug panel was validated through the assessment of drift, precision, and accuracy. The Cocaine/BZE and Opiate Group cutoffs were increased from 30 to 50 ng/mL after several false negative results were obtained during the accuracy portion of the validation. The ELISA assays were validated at two different sites, and the robustness of the method was demonstrated.--Abstract.
dc.identifier.oclc(OCoLC)ocn840817173
dc.identifier.other(AlmaMMSId)9978552385202196
dc.identifier.urihttps://hdl.handle.net/11244/324733
dc.rightsAll rights reserved by the author, who has granted UCO Chambers Library the non-exclusive right to share this material in its online repositories. Contact UCO Chambers Library's Digital Initiatives Working Group at diwg@uco.edu for the permission policy on the use, reproduction or distribution of this material.
dc.subject.lcshForensic sciences
dc.subject.lcshBreast milk
dc.subject.lcshLactation
dc.thesis.degreeM.S., Forensic Science
dc.titleValidation of enzyme-linked immunosorbent assays for the detection of licit and illicit drugs in human breast milk.
dc.typeAcademic theses
thesis.degree.grantorJackson College of Graduate Studies
uco.groupUCO - Graduate Works and Theses::UCO - Theses

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