Nutritional Basis for Colonization Resistance by Human Commensal Escherichia coli Strains HS and Nissle 1917 against E. coli O157:H7 in the Mouse Intestine

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dc.contributor.authorRosalie Maltbyen_US
dc.contributor.authorMary P. Leatham-Jensenen_US
dc.contributor.authorTerri Gibsonen_US
dc.contributor.authorPaul S. Cohenen_US
dc.contributor.authorTyrrell Conwayen_US
dc.date.accessioned2015-01-23T17:17:44Z
dc.date.accessioned2016-03-30T15:31:09Z
dc.date.available2015-01-23T17:17:44Z
dc.date.available2016-03-30T15:31:09Z
dc.date.issued2013-01-17en_US
dc.descriptionThe authors wish to thank Sal Rizzuto for assistance with the animal colonization experiment shown in Figure 4.en_US
dc.descriptionConceived and designed the experiments: RM MPL PSC TC. Performed the experiments: RM MPL TG. Analyzed the data: RM MPL PSC TC. Contributed reagents/materials/analysis tools: RM MPL TG PSC TC. Wrote the paper: RM PSC TC.en_US
dc.description.abstractEscherichia coli is a single species consisting of many biotypes, some of which are commensal colonizers of mammals and others that cause disease. Humans are colonized on average with five commensal biotypes, and it is widely thought that the commensals serve as a barrier to infection by pathogens. Previous studies showed that a combination of three pre-colonized commensal E. coli strains prevents colonization of E. coli O157:H7 in a mouse model (Leatham, et al., 2010, Infect Immun 77: 2876–7886). The commensal biotypes included E. coli HS, which is known to successfully colonize humans at high doses with no adverse effects, and E. coli Nissle 1917, a human commensal strain that is used in Europe as a preventative of traveler's diarrhea. We hypothesized that commensal biotypes could exert colonization resistance by consuming nutrients needed by E. coli O157:H7 to colonize, thus preventing this first step in infection. Here we report that to colonize streptomycin-treated mice E. coli HS consumes six of the twelve sugars tested and E. coli Nissle 1917 uses a complementary yet divergent set of seven sugars to colonize, thus establishing a nutritional basis for the ability of E. coli HS and Nissle 1917 to occupy distinct niches in the mouse intestine. Together these two commensals use the five sugars previously determined to be most important for colonization of E. coli EDL933, an O157:H7 strain. As predicted, the two commensals prevented E. coli EDL933 colonization. The results support a model in which invading pathogenic E. coli must compete with the gut microbiota to obtain the nutrients needed to colonize and establish infection; accordingly, the outcome of the challenge is determined by the aggregate capacity of the native microbiota to consume the nutrients required by the pathogen.en_US
dc.description.peerreviewYesen_US
dc.description.peerreviewnoteshttp://www.plosone.org/static/editorial#peeren_US
dc.identifier.citationMaltby R, Leatham-Jensen MP, Gibson T, Cohen PS, Conway T (2013) Nutritional Basis for Colonization Resistance by Human Commensal Escherichia coli Strains HS and Nissle 1917 against E. coli O157:H7 in the Mouse Intestine. PLoS ONE 8(1): e53957. doi:10.1371/journal.pone.0053957en_US
dc.identifier.doi10.1371/journal.pone.0053957en_US
dc.identifier.urihttp://hdl.handle.net/11244/14089
dc.language.isoen_USen_US
dc.publisherPLos Oneen_US
dc.relation.ispartofseriesPLoS ONE 8(1):e53957en_US
dc.relation.urihttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0053957en_US
dc.rightsAttribution 3.0 United Statesen_US
dc.rights.requestablefalseen_US
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/us/en_US
dc.subjectPLOSen_US
dc.subjectPublic Library of Scienceen_US
dc.subjectOpen Accessen_US
dc.subjectOpen-Accessen_US
dc.subjectScienceen_US
dc.subjectMedicineen_US
dc.subjectBiologyen_US
dc.subjectResearchen_US
dc.subjectPeer-reviewen_US
dc.subjectInclusiveen_US
dc.subjectInterdisciplinaryen_US
dc.subjectAnte-disciplinaryen_US
dc.subjectPhysicsen_US
dc.subjectChemistryen_US
dc.subjectEngineeringen_US
dc.titleNutritional Basis for Colonization Resistance by Human Commensal Escherichia coli Strains HS and Nissle 1917 against E. coli O157:H7 in the Mouse Intestineen_US
dc.typeResearch Articleen_US

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