Development of methodology for the determination of catecholamines, indoleamines, and related hydroxylating enzymes.

dc.contributor.authorWong, Peter Kwei-yen,en_US
dc.date.accessioned2013-08-16T12:28:18Z
dc.date.available2013-08-16T12:28:18Z
dc.date.issued1980en_US
dc.description.abstractThis study was concerned with the development and improvement of analytical procedures using liquid chromatography with electrochemical detection for the determination of activity of tyrosine hydroxylase, tryptophan hydroxylase, and certain individual amines.en_US
dc.description.abstractThe developed methods exhibit sensitivity and selectivity comparable or superior to methods presently in use.en_US
dc.description.abstractA simplified procedure for the determination of 5-hydroxytryptamine, 5-HT, has also been investigated. The analysis time is considerably shortened by eliminating the usual isolation step through the proper choice of homogenization conditions. Sample handling capability is, thus greatly increased.en_US
dc.description.abstractThe neurotransmitters, catecholamines and indoleamines have been investigated extensively during the recent past. A number of assay procedures have been reported for the study of the rate limiting, hydroxylating enzymes as well as the neurotransmitter levels.en_US
dc.description.abstractThe currently reported methods for the determinations of tyrosine hydroxylase and tryptophan hydroxylase focus on the improvement of chromatographic conditions, optimization of reaction parameters and minimization of blank levels.en_US
dc.format.extentxvi, 158 leaves :en_US
dc.identifier.urihttp://hdl.handle.net/11244/4831
dc.noteSource: Dissertation Abstracts International, Volume: 41-10, Section: B, page: 3775.en_US
dc.subjectChemistry, Analytical.en_US
dc.thesis.degreePh.D.en_US
dc.thesis.degreeDisciplineDepartment of Chemistry and Biochemistryen_US
dc.titleDevelopment of methodology for the determination of catecholamines, indoleamines, and related hydroxylating enzymes.en_US
dc.typeThesisen_US
ou.groupCollege of Arts and Sciences::Department of Chemistry and Biochemistry
ou.identifier(UMI)AAI8107969en_US

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