dc.contributor.advisor | Fahlenkamp, Heather | |
dc.contributor.author | Leemasawatdigul, Krisada | |
dc.date.accessioned | 2014-04-16T03:07:27Z | |
dc.date.available | 2014-04-16T03:07:27Z | |
dc.date.issued | 2010-07-01 | |
dc.identifier.uri | https://hdl.handle.net/11244/9645 | |
dc.description.abstract | This research project determined the formation of the static and soluble concentration gradients of monocyte chemoattractant protein-1 (MCP-1) in a collagen matrix using a 3D in vitro vascular tissue model. Stability of MCP-1 and the binding reaction between MCP-1 and collagen were examined and used to develop a mathematical model to estimate the concentration of MCP-1 within the collagen matrix. Effect of storage conditions on the stability of MCP-1 and the effect of a static gradient of MCP-1 on monocyte migration were also investigated in this project. Findings and Conclusions: Recombinant human MCP-1, within a medium containing 10% fetal bovine serum, is stable at standard cell culture conditions (37?C in humidified atmosphere of 5% CO? and 95% air) for seven days. However, storage conditions and duration, as well as the concentration of MCP-1, can affect the stability of MCP-1 when it is stored at working concentrations. Also, it was found that binding reaction between MCP-1 and collagen does exist and is irreversible. Based on these findings, a mathematical model to describe the transport of MCP-1 through the collagen matrix was developed. Results from the mathematical model showed that both static and soluble concentration gradients of MCP-1 are formed in the matrix. The static gradient of MCP-1 was proven to have haptostatic effect on monocyte migration. When the pattern of the static gradient of MCP-1 was taken in to account, this latter finding suggests that the static gradient of MCP-1 may be responsible for attracting high density of monocyte just below the endothelium, as observed in the site of atherosclerosis lesions. | |
dc.format | application/pdf | |
dc.language | en_US | |
dc.publisher | Oklahoma State University | |
dc.rights | Copyright is held by the author who has granted the Oklahoma State University Library the non-exclusive right to share this material in its institutional repository. Contact Digital Library Services at lib-dls@okstate.edu or 405-744-9161 for the permission policy on the use, reproduction or distribution of this material. | |
dc.title | Formation Of Concentration Gradients Of Monocyte Chemoattractant Protein-1 in a Collagen Matrix | |
dc.type | text | |
dc.contributor.committeeMember | Madihally, Sundararajan V. | |
dc.contributor.committeeMember | Ramsey, Joshua D. | |
osu.filename | Leemasawatdigul_okstate_0664M_11010.pdf | |
osu.college | Engineering, Architecture, and Technology | |
osu.accesstype | Open Access | |
dc.description.department | School of Chemical Engineering | |
dc.type.genre | Thesis | |