Effects of Mango and Its Combination with Rosiglitazone on Clinical Parameters in Mice Fed High Fat Diet

Abstract

This study examined the effects of freeze-dried mango (1% or 10% by weight), a fruit rich in vitamins A and C as well as phenolic compounds, in modulating clinical parameters of mice fed high fat (HF) diet. The study also compared the effects of mango to rosiglitazone (50 ppm), a glucose-lowering drug, and determined if addition of mango to a lower dose of rosiglitazone (25 ppm) will have similar effects. Two-month old male C57BL/6 mice were randomly assigned to one of the seven dietary treatment groups (n=8/group) for 60 days: AIN-control (10% fat calories), HF (60% fat calories), HF+1% or 10% mango, HF+50 ppm rosiglitazone, HF+1% mango or 10% mango+25 ppm rosiglitazone. An intraperitoneal glucose tolerance test was performed at 30 and 60 days of treatment. Mice were sacrificed and soft tissues as well as blood were collected after 60 days of treatment. Body composition, clinical parameters (serum HbA1c, plasma glucose, cholesterol, HDL-C, fructosamine, insulin, and triglycerides), and total lipids (i.e. liver and fecal) were determined. Histology was performed on liver and white adipose tissue.