Identification and Expression of Macrophage Migration Inhibitory Factor in Sarcoptes Scabiei
Abstract
The two objectives of this study were: 1) the cloning and sequencing of Macrophage migration inhibitory factor in Sarcoptes scabiei, 2) the cataloging of the existing genes and expressed sequence tags (EST) for Sarcoptes scabiei. Macrophage migration inhibitory factor (MIF) was sequenced from Sarcoptes scabiei, the scabies mite, using RT-PCR and RACE molecular techniques. The resulting nucleotide sequence had a length of 405 base pairs. A partial portion of the 18S ribosomal RNA gene was also sequenced from the scabies mite. The partial 18S nucleotide sequence had a length of 98 nucleotide base pairs. The intial steps for the project resulted in the production of a scabies mite cDNA expression library. A real time (qPCR) assay was performed with MIF from scabies mites and various tick species. The results showed that the scabies MIF was expressed three times more than that of the control of Dermacentor variabilis salivary gland MIF and 1.3 times that of D.variabilis midgut MIF. Finally, a catalogue of S. scabiei partial and full length gene sequences and expressed sequence tags (ESTs) was compiled to assess what is known about S. scabiei at the level of gene expression and to provide context for research.
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- OSU Theses [15752]