Effect of Site of Infusion of Lactobacillus acidophilus and Propionibacterium freudenreichii on Production and Nutrient Digestibility in Lactating Dairy Cows
Thompson, Kyle Samuel
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The objective was to evaluate site of infusion of 10^9 CFU/g of Lactobacillus acidophilus and 10^ 9 CFU/g Propionibacterium freudenreichii (DFM) on DMI, rumen kinetics, ruminal VFA, digestibility, milk production, milk components, and blood metabolites in lactating dairy cows. A total of 4 Holstein cows equipped with ruminal cannulas were used in Latin square design experiment with 4 periods. Each period consisted of 14 d of no treatment to prevent crossover contamination, 14 d of adaptation to treatments, 8 d of sampling, and 1 d for ruminal evacuations for a total of 37 d. Within each period, cows were assigned to 1 of 4 treatments: 1) cows fed a TMR formulated to meet or exceed nutrient requirements plus 5 g of lactose twice daily without the addition of direct fed microbial (CONTROL); 2) cows fed the TMR with a daily dose of DFM top dressed on the feed twice daily 3) cows fed the TMR with ruminal infusion of the DFM administered twice daily (RI); and 4) cows fed the TMR plus abomasal infusion of the DFM twice daily (PRI). During the sampling period within each period, DMI and milk production were measured daily with set days for blood, and rumen fluid collection. Data were analyzed using the MIXED procedure of SAS with animal within period as a random effect. DMI was not different between treatments. No difference in rumen kinetics, pH, VFA (acetate, propionate, butyrate, and acetate/propionate ratio), ammonia, or digestibility. There was no difference in milk production (P > 0.87), butter fat (P > 0.21), milk protein (P > 0.83), lactose (P > 0.91), SNF (P > 0.88) , MUN (P > 0.49), and SCC (P > 0.54). No difference in most of the milk fatty acids except for 8:0 which had a greater concentration (P > 0.01) in PRI over control, TD, and RI. Route of administering DFM overall had no effects on DMI, rumen kinetics, ruminal VFA, digestibility, milk production or milk components in the present experiment.
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