Modulation of the transcriptional control of the carbon concentration mechanism within Synechocystis sp. PCC 6803

Abstract

Scope and Method of Study:

This study involved looking at paralogous proteins (CcmR and CmpR), which function as transcriptional regulators of the carbon concentration mechanism within the cyanobacteria Synechocystis sp. PCC 6803. Techniques used include electrophoretic mobility shift assay, Dynamic Light Scattering, gel-filtration chromatography, and surface plasmon resonance.

Findings and Conclusions:

For the first time the identification of the ligand molecules for CcmR, NADP+ and a-KG, and their subsequent modulation of the binding characteristics for the protein and to target DNA. Additionally, the ligand molecules have been identified for CmpR, 2PG and RuBP. This allows for the first time the construction of a regulatory network or pathway for the carbon concentration mechanism and the apparent in vivo small molecule regulators.