17β-HSD13 has sex-based differential expression in Hepatitis C virus-induced cirrhosis and hepatocellular carcinoma
Date
2023-02-17Author
Deniega, Carldon
Pathak, Sachi
Swope, Logan
Hajimirsadeghi, Dorsa
Pande, Radhika
Eslinger, Christy
Platt, Anna
Das, Subhas
Zhao, Daniel
Kaul, Anil
Kaul, Rashmi
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Background: Sex-based differences are observed in chronic hepatitis C virus (HCV) infections leading to cirrhosis and hepatocellular carcinoma (HCC). We previously showed that liver estrogen receptor (ER-) mediated sex-based differences exist in cirrhosis and HCC. Liver ER-binding may lead to protective effects in pre-menopausal women. This study aimed to determine sex-based differential role of 17βHSD13 in development of cirrhosis and HCC. We hypothesized that chronic HCV infection leads to dysregulated 17β-HSD13 in male cirrhosis and progression to HCC. Methods: 65 (normal, cirrhosis, HCC) liver tissues were obtained from NIH Liver Tissue Bank. DIA proteomics mapped 4445 proteins, including 17β-HSD13. Clinical correlation with bilirubin, AST, ALP, and creatinine was determined (spearman’s). Immunohistochemistry validated 17β-HSD13 protein expression in tissues. Results: 17β-HSD13 had significantly lower expression in male cirrhosis group than females (P<0.05). In contrast, 17β-HSD13 expression in normal males was significantly greater than normal females (P<0.05). In HCC group, the expression in males was down-regulated compared to HCC females (P<0.05). Bilirubin values showed negative correlation with 17β-HSD13 expression (P<0.05) between cirrhosis and HCC (males alone and combined sex data). Conclusions: Low 17β-HSD13 levels may predict worse disease in males with cirrhosis or HCC serving as disease biomarker. This novel report shows sex-based differences in 17β-HSD13 during HCV-induced cirrhosis development.
Citation
Deniega, C., Pathak, S., Swope, L., Hajimirsadeghi, D., Pande, R., Eslinger, C., Platt, A., Das, S., Zhao, D., Kaul, A., and Kaul, R. (2023, February 17). 17β-HSD13 has sex-based differential expression in Hepatitis C virus-induced cirrhosis and hepatocellular carcinoma. Poster presented at Research Week, Oklahoma State University Center for Health Sciences, Tulsa, Ok.
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- Research Week 2023 [83]