CELLULASE6 and MANNANASE7 affect cell differentiation and silique dehiscence in Arabidopsis
Date
2017-06-19Author
Yang, Ming
He, Hanjun
Bai, Mei
Hu, Yanting
Wu, Hong
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Cellulases, hemicellulases and pectinases play important roles in fruit development and maturation, but mutants with defects in the fruit have not been reported for cellulase or hemicellulase genes. Here we report the functional characterization of cellulase gene CEL6 and hemicellulase gene MAN7 in silique development and dehiscence in Arabidopsis. These genes were found to be expressed in vegetative and reproductive organs, and their expression in the silique partially depended on the IND and ALC transcriptional factors. Mutant alleles of cel6 and man7 exhibited delayed secondary cell wall thickening and altered cell morphology in the valve margin and impaired silique dehiscence. Cells in the separation layer in nearly mature siliques of the single mutants and the cel6-1 man7-3 double mutant remained intact whereas they degenerated in the wild-type control. Phenotypic studies of single, double, triple and quadruple mutants revealed that the higher-order mutant combinations of the cel6-1, man7-3, and pectinase adpg1-1 and adpg2- 1 mutations produced more severe silique indehiscent phenotypes than the corresponding lower-order mutant combinations, except for some combinations involving cel6-1, man7-3, and adpg2-1. Our results demonstrate that the ability of the silique to dehisce can be manipulated to different degrees by altering the activities of proteins of different types.
Citation
He, H., Bai, M., Hu, Y., Wu, H., Yang, M., (2017). CELLULASE6 and MANNANASE7 affect cell differentiation and silique dehiscence in Arabidopsis.