Shiga toxin 2 overexpression in Escherichia coli O157:H7 strains associated with severe human disease
Date
2011-12Author
Neupane, Mahesh
Abu-Ali, Galeb S.
Mitra, Avishek
Lacher, David W.
Manning, Shannon D.
Riordan, James T.
Metadata
Show full item recordAbstract
Variation in disease severity among Escherichia coli O157:H7 infections may result from differential expression of Shiga toxin 2 (Stx2). Eleven strains belonging to four prominent phylogenetic clades, including clade 8 strains representative of the 2006 U.S. spinach outbreak, were examined for stx2 expression by real-time PCR and western blot analysis. Clade 8 strains were shown to overexpress stx2 basally, and following induction with ciprofloxacin when compared to strains from clades 1-3. Differences in stx2 expression generally correlated with Stx2 protein levels. Single-nucleotide polymorphisms identified in regions upstream of stx2AB in clade 8 strains were largely absent in non-clade 8 strains. This study concludes that stx2 overexpression is common to strains from clade 8 associated with hemolytic uremic syndrome, and describes SNPs which may affect stx2 expression and which could be useful in the genetic differentiation of highly-virulent strains.
Citation
Neupane, M., Abu-Ali, G. S., Mitra, A., Lacher, D. W., Manning, S. D., Riordan, J. T. (2011). Shiga toxin 2 overexpression in Escherichia coli O157:H7 strains associated with severe human disease. Microbial Pathogenesis, 51(6), pp. 466-470. https://doi.org/10.1016/j.micpath.2011.07.009
Relation URI
https://www.ncbi.nlm.nih.gov/pubmed/21864671http://dx.doi.org/10.1016/j.micpath.2011.07.009