Enzymatic assessment of cells with distinct TP53 mutations

Abstract

The p53 transcription factor, encoded by the human gene TP53, is the most commonly mutated gene in human cancers. Once the TP53 gene is mutated, p53 proteins not only lose their tumor suppression abilities but can also contribute to tumor growth. One-way mutant p53 can do this is by increasing iron uptake and availability by altering the regulation of iron-sulfur (Fe-S) cluster containing proteins. Understanding such alterations are important because Fe-S cluster proteins play a role in both energy metabolism and DNA repair enzymes, which can suppress the progression and expansion of cancerous tumors. This study’s main focus was to examine how TP53 mutation status influences Fe-S cluster protein regulation, and subsequently iron homeostasis in tumor cells. First, we assessed the activity of the Fe-S containing enzymes cytosolic and mitochondrial aconitase in cell lines expressing a variety of TP53 mutations. Specifically, we will use cell lines that express the six most commonly observed TP53 mutations, which represent nearly 25% of all TP53 mutations in human cancers. We found that while aconitase activity decreased as expected following the removal of iron in cells expressing WTP53, the influence of iron chelation on aconitase activity in cells expressing mutant TP53 varied depending on the mutation type. This data strongly suggests that TP53 mutations significantly influence how the activity of Fe-S cluster enzymes is regulated. Thus, our ongoing studies are focused on investigating how TP53 mutation status influences the regulation of genes involved with Fe-S cluster biogenesis and consequently, Fe-S cluster containing protein activity.