This study examined the Wyche Farm-1 core and selected depths of interest for biomarker analysis. Previously employed methods for biomarker characterization were slow and labor-intensive using the gas chromatography mass spectrometer (GCMS), but this study utilizes a new method for the liquid chromatography mass spectrometer (LCMS) that requires minimal time and labor to produce comparable results. Applying this novel LCMS method, the previously undetected polar biomarkers, such as metalloporphyrins and newly identified tocopherol derivatives, are characterized in this study. Comparing results of aromatic carotenoids generated by both GCMS and LCMS yields, a general consistency in composition and depth profile, thus validating the new characterization method. However, due to the different procedures of sample preparation applied and standards used for quantification, the calculated absolute concentrations of aromatic carotenoids are two orders of magnitude higher for the GCMS method. Metalloporphyrin analyses indicates an unexpected high nickel porphyrin abundance during the euxinic Middle Woodford Shale evidenced by the distribution of aromatic carotenoids. This unexpected spike in nickel porphyrin concentration reflects a possible metal chelation preference related to the different molecular structures of BiCAP, DPEP and ETIO porphyrins, calling into question their use as reliable redox proxies. The MTTCs have been proposed as a salinity proxy in previous studies. However, our data show they possibly correlate to redox. The distribution of tocopherol derivatives likely reflects the population dynamics of phytoplankton resulting from the evolution of water chemistry during this time interval.